Partial purification and characterization acidophilic lipase from Bacillus cereus ALP E1

Area of Science:

• Microbiology
• Enzymology
• Biotechnology

Background:

• Microbial lipases are vital for industrial processes.
• Bacillus cereus is a known lipase producer, but exploration is limited.
• Novel lipases are needed for diverse applications.

Purpose of the Study:

• To isolate and purify lipase from Bacillus cereus ALP E1.
• To characterize the biochemical properties of the enzyme.
• To assess its potential for industrial use.

Main Methods:

• Isolation of Bacillus cereus ALP E1 from seawater.
• Submerged fermentation for lipase production.
• Partial purification using ammonium sulphate fractionation and dialysis.
• Characterization of enzyme activity, pH, temperature, and substrate specificity.
• Analysis using SDS-PAGE.

Main Results:

• Partially purified lipase with 12.31-fold purification and specific activity of 31083.14 U/mg.
• SDS-PAGE indicated a molecular weight of approximately 55 kDa.
• Optimal activity at pH 5 and 40°C, with preference for p-nitrophenyl stearate (C18).
• Enzyme activity influenced by divalent cations (Ba2+, Mg2+), surfactants (Tween 20, Tween 80), and organic solvents (chloroform).

Conclusions:

• Seawater is a valuable source for discovering highly active lipases.
• The characterized lipase from Bacillus cereus ALP E1 exhibits properties suitable for industrial applications.
• Further research can optimize its use in various sectors.