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Development of a high-yield Rabbit line for enhanced animal pharming.

Jun Song1, Dongshan Yang1, Lingjie Kong2

  • 1Center for Advanced Models for Translational Sciences and Therapeutics, University of Michigan Medical Center, Ann Arbor, MI, 48109, USA.

Biological Research
|December 10, 2025
PubMed
Summary
This summary is machine-generated.

Researchers enhanced recombinant protein production in transgenic rabbits using a novel gene editing technique. This method significantly increased yields, offering a more efficient approach to animal pharming for drug development.

Keywords:
Animal pharmingCRISPR/Cas9CSN2 gene promoterRabbits

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Area of Science:

  • Biotechnology
  • Genetics
  • Animal Pharming

Background:

  • Animal pharming utilizes transgenic animals for recombinant protein drug production.
  • Current methods using conventional transgenesis in rabbits yield low protein levels (1-2 g/L).
  • Challenges include unpredictable success rates and uncontrolled transgene integration.

Purpose of the Study:

  • To enhance recombinant protein yield in transgenic rabbits.
  • To investigate the use of a native milk protein gene promoter for transgene expression.
  • To develop a more efficient animal pharming strategy.

Main Methods:

  • Generated knock-in rabbits using CRISPR/Cas9 and RS-1 technology.
  • Utilized the CSN2 gene promoter to drive tdTomato expression.
  • Produced heterozygous and homozygous knock-in offspring.

Main Results:

  • Achieved significantly higher recombinant protein yields.
  • Homozygous rabbits produced 15-20 g/L of tdTomato.
  • Demonstrated successful transmission of the knock-in allele.

Conclusions:

  • Using the CSN2 promoter enhances recombinant protein production in rabbits.
  • CRISPR/Cas9-mediated knock-in with RS-1 is effective for generating high-yield pharming animals.
  • This strategy offers a promising advancement for pharmaceutical production in transgenic rabbits.