Characterization of Terpenoids in Aromatic Plants Using Raman Spectroscopy and Gas Chromatography-Mass Spectrometry
Milagros Granda-Santos1,2,3, Katherine Reyna-Gonzales2, Llisela Torrejón-Valqui2
1Programa Doctoral en Ciencias para el Desarrollo Sustentable, Escuela de Posgrado, Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas, Chachapoyas 01001, Peru.
Abstract:
The study characterized the essential oils of eight aromatic medicinal plants (Tagetes filifolia, Aloysia citrodora, Cymbopogon citratus, Eucalyptus globulus, Chamaemelum nobile, Piper aduncum, Minthostachys mollis, and Rosmarinus officinalis) using Raman spectroscopy and gas chromatography-mass spectrometry (GC-MS). Raman spectra allowed the identification of bands associated with C-H, C=C, C-O, and C=O bonds, evidencing the presence of monoterpenes, sesquiterpenes, and oxygenated compounds. GC-MS analysis confirmed these results, detecting 224 compounds, predominantly terpenoids. Among the major compounds, cis,cis-nepetalactone (30.16%), β-caryophyllene (up to 18.26%), citronellol (10.92%), citral, and linalool were found. The combination of both techniques made it possible to relate the chemical composition to the molecular structure. This showed that the differences between species are mainly due to the proportion of oxygenated compounds (citral, linalool, geraniol) compared with aromatic hydrocarbons (β-caryophyllene, D-limonene, β-pinene). Additionally, the presence of cis,cis-nepetalactone in M. mollis was reported for the first time, representing a significant chemical finding.
More Related Videos
11:14Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
Published on: October 2, 2016
08:43PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
Published on: May 11, 2017
Related Concept Videos
Mass Spectrometry: Aromatic Compound Fragmentation
Raman Spectroscopy: Overview
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
NMR Spectroscopy of Aromatic Compounds
Aromatic Compounds: Overview
In 1825, Faraday isolated...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Gas Chromatography–Mass Spectrometry (GC–MS)
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
