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Isolation and Characterization of Extracellular Vesicles Derived from Mango Fruits
Aleksandra Steć1, Grzegorz Szaknis1, Anna Skowrońska1
1Department of Toxicology, Faculty of Pharmacy, Medical University of Gdansk, 107 Hallera Street, 80-416 Gdansk, Poland.
International Journal of Molecular Sciences
|December 11, 2025
Summary
Researchers isolated extracellular vesicles (EVs) from mango pulp, identifying 1084 proteins with antioxidant and anti-inflammatory functions. These mango EVs may explain some health benefits of consuming the fruit.
Area of Science:
- Plant biology
- Biochemistry
- Nanotechnology
Background:
- Mango (Mangifera indica L.) is a tropical fruit valued for its rich content of carotenoids, polyphenols, and vitamins.
- These compounds contribute to mango's nutritional and medicinal properties, yet their role in fruit-derived extracellular vesicles (EVs) remains unexplored.
- Extracellular vesicles (EVs) are increasingly recognized for their roles in intercellular communication and biological functions.
Purpose of the Study:
- To isolate and characterize extracellular vesicles (EVs) from mango fruit pulp.
- To identify proteins within mango-derived EVs and assess their potential biological functions.
- To establish a foundation for understanding the contribution of mango EVs to the fruit's health benefits.
Main Methods:
- A multi-step isolation workflow was developed, combining differential centrifugation, filtration, and size-exclusion chromatography.
- The International Society of Extracellular Vesicles (ISEV) guidelines were followed for characterization of the isolated EVs.
- Proteomic analysis was performed to identify proteins present in the mango EV fraction.
Main Results:
- A high-quality EV fraction was successfully isolated from mango pulp using optimized size-exclusion chromatography with Sepharose CL-6B beads.
- Physicochemical properties of the mango EVs were characterized.
- Proteomic analysis identified 1084 proteins, including numerous proteins associated with antioxidant, antimicrobial, and anti-inflammatory activities.
Conclusions:
- This study presents the first comprehensive characterization of extracellular vesicles (EVs) derived from mango fruit.
- The identified proteins suggest that mango EVs play a role in the fruit's biological activities.
- Mango EVs represent a novel area of research for understanding the health benefits of mango consumption.
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