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Updated: Jan 9, 2026
00:08
A Rapid In Vivo Bioassay for Developmentally Active Enhancers
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Interaction Between Enhancers and Promoters in Chicken Genome.
Valentina A Grushina1, Anastasia P Filatova2, Valeria S Gagarina1
1Department of Computational Biology, Sirius University of Science and Technology, 354340 Sirius, Russia.
International Journal of Molecular Sciences
|December 11, 2025
Summary
Gene expression is linked to genomic enhancers within topologically associating domains (TADs). This study found a significant correlation between enhancer activity and gene expression in chickens, highlighting enhancer roles in gene regulation.
Area of Science:
- Genomics
- Molecular Biology
- Gene Regulation
Background:
- Gene expression is regulated by promoter-enhancer interactions within topologically associating domains (TADs).
- Enhancer activity can be quantified by measuring enhancer RNAs (eRNAs).
- The CAGE (Cap Analysis of Gene Expression) method allows simultaneous assessment of promoter and enhancer activity.
Purpose of the Study:
- To investigate the correlation between gene expression and enhancer activity within TADs across different chicken tissues.
- To evaluate the biological significance of genes regulated by enhancers.
Main Methods:
- Analysis of gene and enhancer activity correlations within TADs.
- Utilizing CAGE methodology for simultaneous transcriptional profiling.
- Comparative analysis in slow- and fast-growing chicken breeds across multiple tissues.
Main Results:
- A statistically significant association was observed between gene expression levels and enhancer activity in all examined tissues.
- Enhancer-mediated regulation was found to activate key pathways, including transcriptional control and nucleic acid biosynthesis.
Conclusions:
- Enhancer activity is significantly correlated with gene expression levels across various chicken tissues.
- Enhancers play a crucial role in regulating gene expression, particularly in pathways related to transcription and nucleic acid synthesis.
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