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Constitutive OsCIN1 Expression Reprograms Source-Sink Dynamics and Compromises Agronomic Traits in Rice.

Cong Danh Nguyen1, Joon-Seob Eom2, Jung-Il Cho2

  • 1Division of Life Science, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Republic of Korea.

International Journal of Molecular Sciences
|December 11, 2025
PubMed
Summary
This summary is machine-generated.

Overexpressing cell wall invertase 1 (OsCIN1) in rice disrupted sugar gradients, leading to reduced tillers and grain weight, demonstrating precise spatial control of enzymes is crucial for yield.

Keywords:
OsCIN1carbon partitioningcell wall invertasericesink strengthyield

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Area of Science:

  • Plant Biology
  • Biochemistry
  • Agricultural Science

Background:

  • Cell wall invertases (CINs) regulate photoassimilate allocation by establishing sucrose gradients.
  • OsCIN1 and OsCIN2 are identified as key regulators of sink strength in rice.
  • Understanding CIN activity is crucial for optimizing crop yield.

Purpose of the Study:

  • To investigate the effect of increased OsCIN1 activity on rice grain yield.
  • To determine if enhancing CIN activity can improve sink strength and productivity.
  • To elucidate the role of OsCIN1 in carbon partitioning and agronomic traits.

Main Methods:

  • Generation of OsCIN1 overexpression (OX) rice lines using the CaMV 35S promoter.
  • Subcellular localization of OsCIN1-GFP and promoter::GUS expression analysis.
  • Sugar profiling of flag leaves and field evaluation of agronomic traits (tillers, 1000-grain weight).

Main Results:

  • OsCIN1-GFP confirmed apoplastic localization; promoter activity observed in vascular tissues, particularly during seed development.
  • Elevated CIN activity led to sucrose depletion in flag leaves, with increased hexose and starch accumulation.
  • OsCIN1 OX plants showed ~50% fewer tillers, reduced 1000-grain weight, and overall decreased productivity compared to wild type (WT).

Conclusions:

  • Ectopic OsCIN1 expression disrupts sucrose gradients, weakens carbon partitioning to sink tissues, and impairs key agronomic traits.
  • Precise spatial and temporal control of CIN activity is essential for effective carbon flux and yield enhancement.
  • Targeting CINs requires careful consideration of their spatiotemporal expression patterns to avoid negative impacts on crop productivity.