Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...

Non-LTR Retrotransposons

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The inhibition of pathological IgE in allergic diseases by natural compounds.

Natural products and bioprospecting·2026

Same author

Comparative Dosimetric and Radiobiological Evaluation of Dental Structures and Normal Tissue Complication Probability.

Cureus·2026

Same author

Understanding Infantile Atopic Dermatitis: A Review of Environmental, Familial, Genetic and Microbial Influences.

Current allergy and asthma reports·2026

Same author

Cross-IgE sensitization correlates with cross-anaphylaxis among peanut and multiple tree nuts in a murine model.

Frontiers in allergy·2026

Same author

Targeting the Tumor Microenvironment in Triple-Negative Breast Cancer: Emerging Roles of Monoclonal Antibodies and Immune Modulation.

Cancers·2026

Same author

B cell development and longevity of IgE plasma cells.

Allergology international : official journal of the Japanese Society of Allergology·2026

Search research articles

Related Experiment Video

Updated: Jan 9, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

Long Non-Coding RNA DUXAP10 Promotes Tumorigenesis and Metastasis in Anaplastic Thyroid Cancer.

Nicole R DeSouza¹, Michelle Carnazza¹, Tara Jarboe¹

¹Department of Pathology, Microbiology & Immunology, New York Medical College, Valhalla, NY 10595, USA.

|December 11, 2025

Summary

Long non-coding RNA DUXAP10 drives anaplastic thyroid cancer (ATC) progression. Inhibiting DUXAP10 reduced tumor growth and metastasis, identifying it as a potential therapeutic target for this lethal cancer.

Keywords:

RNA therapeutics cancer long non-coding RNAs miRNAs regulatory non-coding variants ribodiagnostics targeted therapy

More Related Videos

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Published on: February 3, 2023

Related Experiment Videos

Last Updated: Jan 9, 2026

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Spontaneous Murine Model of Anaplastic Thyroid Cancer

Published on: February 3, 2023

Area of Science:

Molecular Biology
Genomics
Cancer Research

Background:

Long non-coding RNAs (lncRNAs) regulate gene expression and impact cancer development.
Anaplastic thyroid cancer (ATC) is an aggressive and lethal malignancy with poorly understood molecular mechanisms.
Understanding lncRNA dysregulation in ATC is crucial for identifying new therapeutic targets.

Purpose of the Study:

To investigate the role of lncRNA Double Homeobox A Pseudogene 10 (DUXAP10) in anaplastic thyroid cancer.
To determine if DUXAP10 is a potential therapeutic target for ATC.

Main Methods:

Analysis of patient genomic datasets to identify upregulated lncRNAs in ATC.
CRISPR-interference (CRISPRi) technology to transcriptionally repress DUXAP10 expression in ATC cell lines.
In vitro and in vivo experiments to assess the functional impact of DUXAP10 on ATC phenotypes.

Main Results:

DUXAP10 was found to be significantly upregulated in ATC compared to normal thyroid tissue.
Repression of DUXAP10 using CRISPRi markedly reduced ATC cell proliferation, viability, clonogenicity, invasion, and migration.
DUXAP10 inhibition also suppressed in vivo tumor growth and metastasis in preclinical models.

Conclusions:

DUXAP10 plays a critical role in promoting the aggressive phenotypes of anaplastic thyroid cancer.
DUXAP10 serves as a potential prognostic marker for ATC.
DUXAP10 represents a promising therapeutic target for combating ATC progression.