Related Experiment Video
Updated: Jan 9, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
LINC02081 as an oncogene promotes cell proliferation and G2/M cycle through p53 signaling pathway in lung
Lizhong Zeng1, Haimei Wen1, Tong Jiao1
1Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Background:
LINC02081 is a newly identified long non-coding RNA (lncRNA) with an uncharacterized biological function. This study aimed to investigate the role of LINC02081 in lung adenocarcinoma (LUAD).
Methods:
Bioinformatics analysis utilizing The Cancer Genome Atlas (TCGA) database assessed LINC02081 expression and its clinical significance. Nuclear and cytoplasmic RNA fractionation detected the subcellular localization of LINC02081. Cell proliferation was evaluated using Cell Counting Kit-8 (CCK-8) assays, 5-ethynyl-2'-deoxyuridine (EdU) assays, and colony formation assays. Flow cytometry analyzed the cell cycle and apoptosis. p53 expression was assessed via immunofluorescence, while p21 messenger RNA (mRNA) levels were analyzed by reverse transcription quantitative polymerase chain reaction (RT-qPCR).
Results:
LINC02081 was highly expressed in LUAD tissues, both in the TCGA dataset and our clinical samples. Elevated LINC02081 expression correlated positively with poor prognosis and advanced clinical stage. LINC02081 localized predominantly to the nucleus. Gene enrichment analysis revealed significant associations between LINC02081 and both the cell cycle and p53 signaling pathway. Successful knockdown of LINC02081 using antisense oligonucleotides (ASOs) significantly suppressed LUAD cell proliferation, inhibited G2/M phase progression, and promoted cell apoptosis. Knockdown of LINC02081 promoted p53 and p21 expression.
Conclusions:
Our findings suggest that LINC02081 promotes the malignant progression of LUAD. This effect may involve the p53 signaling pathway. This study provides novel insights into LUAD pathogenesis and indicates LINC02081 may be a potential therapeutic target.
More Related Videos
13:18Network Pharmacology Prediction and Experimental Validation of Trichosanthes-Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
Published on: March 3, 2023
11:15Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Published on: September 20, 2016
Related Concept Videos
Abnormal Proliferation
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
PI3K/mTOR/AKT Signaling Pathway