Circ_0111277 impedes cell proliferation, migration, and invasion of trophoblast cell by modulating miR-411-5p/GADD45A...

Area of Science:

• Molecular Biology
• Genetics
• Cell Biology

Background:

• Circular RNAs (circRNAs) are key regulators in various diseases, including preeclampsia (PE).
• This study investigates the role of circ_0111277 in trophoblast cells, which are critical for placental development and function.
• Preeclampsia is a serious pregnancy complication with significant maternal and fetal health implications.

Purpose of the Study:

• To elucidate the function of circ_0111277 in the context of preeclampsia.
• To determine the molecular mechanism by which circ_0111277 affects trophoblast cell behavior.
• To explore the regulatory relationship between circ_0111277, microRNA-411-5p (miR-411-5p), and GADD45A in PE.

Main Methods:

• Quantitative real-time PCR and Western blot were used to measure the expression levels of circ_0111277, miR-411-5p, and GADD45A.
• Cell proliferation, migration, and invasion were assessed using CCK-8, colony formation, EdU, transwell, and wound healing assays.
• Dual-luciferase reporter assays were employed to confirm the direct binding interactions between miR-411-5p and its targets, circ_0111277 and GADD45A.

Main Results:

• Circ_0111277 and GADD45A expression were elevated, while miR-411-5p was downregulated in preeclampsia.
• Knockdown of circ_0111277 significantly inhibited trophoblast cell proliferation, migration, and invasion.
• Inhibition of miR-411-5p reversed the effects of circ_0111277 knockdown, and miR-411-5p mimic suppressed cell growth by regulating GADD45A.

Conclusions:

• Circ_0111277 promotes preeclampsia progression by sponging miR-411-5p and subsequently upregulating GADD45A expression.
• The circ_0111277/miR-411-5p/GADD45A axis represents a potential therapeutic target for preeclampsia.
• Understanding this regulatory pathway provides insights into the molecular pathogenesis of preeclampsia.