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Updated: Jan 7, 2026
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
NiCl2@g-C3N4-based Label-free Dual-mode Sensing Platform for Highly Sensitive and Selective Detection of
Jinxiong Xue1, Jingping Huang2, Xue Lin2
1Department of Urology, Fuzhou No. 1 Hospital Affiliated with Fujian Medical University, 350009, Fuzhou, China.
Abstract:
MicroRNA-183-5p is a clinically relevant biomarker, yet its detection is hindered by short sequence length, high homology, and low abundance in complex biological fluids. We report a hydrothermally synthesized nickel chloride-decorated graphitic carbon nitride (NiCl2@g-C3N4) with intrinsic peroxidase-like and fluorescence activities for label-free dual-mode detection. Characterized by multiple spectroscopic and microscopic techniques, the nanocomposite enables quantitative analysis via π-π stacking and van der Waals interactions between the probe and target, eliminating the need for enzymes or fluorescent labels. Under optimal conditions, using the formula LOD = 3Sb/b, the detection limit was calculated as 0.69. This material with broad linear ranges, excellent selectivity, month-long stability, and recovery rates of 97.7-101.6% in spiked serum. This facile and cost-effective platform offers a promising tool for sensitive miRNA quantification in clinical diagnostics.
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