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Protocol for Dengue Infections in Mosquitoes A. aegypti and Infection Phenotype Determination
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Emergence of Dengue Virus Serotype 3, Lineage III_B.3.2, Angola

Jocelyne Neto de Vasconcelos, Ingra M Claro, Raissa Heloisa de Araujo Eliodoro

    Emerging Infectious Diseases
    |December 11, 2025
    PubMed

    Insights

    Dengue virus serotype 3 was found in 11.8% of patients in Angola. Genetic analysis suggests it was imported from the Americas and spread locally.

    Area of Science:

    • Medical Entomology
    • Virology
    • Epidemiology

    Background:

    • Dengue virus (DENV) poses a significant global health threat.
    • DENV serotype 3 (DENV-3) has re-emerged in various regions.
    • Limited data exists on DENV circulation in Angola.

    Purpose of the Study:

    • To investigate the presence and genetic characteristics of dengue virus in Luanda Province, Angola.
    • To determine the origin and transmission dynamics of detected DENV-3 strains.

    Main Methods:

    • Detection of dengue virus in febrile patients using molecular methods.
    • Phylogenetic analysis of viral sequences to determine lineage and origin.

    Main Results:

    • Dengue virus serotype 3 (DENV-3) was detected in 11.8% (16/136) of febrile patients.
    • Genetic analysis identified the DENV-3 lineage as III_B.3.2.
    • Evidence suggests introduction from the Americas in late 2022, followed by local transmission.

    Conclusions:

    • DENV-3 is actively circulating in Luanda Province, Angola.
    • The identified lineage points to a probable introduction from the Americas.
    • Understanding transmission patterns is crucial for public health interventions.
    Keywords:
    Angolaarbovirusdengue virusepidemiologyflavivirusmobilitymosquito-borne infectionsserotype 3suitabilitysurveillancevector-borne infectionsviruses

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