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Mosquito sex separation using complementation of selectable traits and engineered neo-sex chromosomes
Doron S Y Zaada1, Or Toren1, Flavia Krsticevic1
1Department of Entomology, Institute of Environmental Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel.
Nature Communications
|December 11, 2025
Summary
Scientists developed a new method for mosquito genetic control using CRISPR technology. This platform creates genetically modified mosquitoes for effective sex separation, aiding in pest control strategies.
Area of Science:
- Genetics
- Entomology
- Molecular Biology
Background:
- Effective sex separation is crucial for mosquito genetic control strategies.
- Genetic sexing strains (GSS) facilitate female mosquito removal by linking maleness to selectable traits.
Purpose of the Study:
- To develop a versatile platform for creating GSS in Aedes albopictus.
- To integrate CRISPR-engineered selectable phenotypes with sex conversion using the nix gene.
Main Methods:
- Utilized CRISPR technology to disrupt the yellow pigmentation gene.
- Restored gene function in males via nix-containing transgenes for sex-specific traits.
- Integrated engineered traits with natural dimorphisms for enhanced separation.
Main Results:
- Developed a stable Aedes albopictus strain with yellow females and dark males.
- Demonstrated that the yellow phenotype enhances protandry-based sorting and reduces accidental female release.
- Showcased compatibility with size-based separation for improved accuracy.
Conclusions:
- Established the first engineered sex-linked selectable trait in mosquitoes based on endogenous genes.
- Created a foundation for scalable GSS development in Aedes albopictus.
- The developed platform offers a promising tool for effective mosquito population control.
