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Development of a Komagataella phaffii-based expression system for high-activity anti-SARS-CoV-2 neutralizing

Xueqin Liu1,2,3,4,5, Qiaoli Lang1,2,3,4,5, Nan Huang1,2,3,4,5

  • 1Chongqing Academy of Animal Sciences, Chongqing, China.

AMB Express
|December 11, 2025
PubMed
Abstract

No abstract available in PubMed .

Keywords:
Komagataella phaffiiPichia pastorisAntibodyFermentation optimizationSARS-CoV-2scFv

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Hybridoma Technology01:31

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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