Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR activation may...
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
Spanning Openings in Brick Walls01:20

Spanning Openings in Brick Walls

In brick wall construction, supporting structures are crucial for openings like windows and doors to maintain the integrity and support the weight of the wall above. These supports include lintels, corbels, and arches, each serving specific structural purposes.
Lintels are primary supports used to span openings and can be crafted from materials such as reinforced concrete, steel-reinforced brick masonry, or simple steel angles. These are straightforward to install and are typically concealed...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic Interface Engineering via Buffer Layer and UVO Treatment for High-Performance PbS Quantum Dot Near-Infrared Photodiodes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Increased serum phenylalanine and tyrosine concentration related to inflammation in patients with primary angiitis of the central nervous system.

Biochemistry and biophysics reports·2026
Same author

Health risk of PM<sub>2.5</sub>-bound heavy metals in a megacity in South China: Comparison between before and after the outbreak of COVID-19.

Journal of environmental sciences (China)·2026
Same author

SEC-MALS analysis of polydeoxyribonucleotides: Revealing product heterogeneity and enabling measurement standardization.

Talanta·2026
Same author

The Motor Neuron Disease Register for England, Wales, and Northern Ireland: Protocol for a Population Register.

JMIR research protocols·2026
Same author

Celebrating a breakthrough for amyotrophic lateral sclerosis.

Brain : a journal of neurology·2026

Related Experiment Video

Updated: May 16, 2026

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
10:53

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Published on: September 15, 2014

15.6K

Targeted BDNF upregulation via upstream open reading frame disruption.

Ning Feng1, Thomas Goedert1, Nenad Svrzikapa2

  • 1Institute of Developmental and Regenerative Medicine, University of Oxford, IMS-Tetsuya Nakamura Building, Old Road Campus, Roosevelt Dr, Headington, Oxford OX3 7TY, UK; Department of Paediatrics, University of Oxford, Roosevelt Dr, Headington, Oxford OX3 7TY, UK.

Molecular Therapy : the Journal of the American Society of Gene Therapy
|December 12, 2025
PubMed
Summary

Upstream open reading frames (uORFs) in brain-derived neurotrophic factor (BDNF) transcripts regulate protein output. Disrupting a single uORF start codon via base editing increased BDNF protein levels, offering a potential therapeutic strategy.

Keywords:
BDNFbase editingbrain-derived neurotrophic factoruORFupstream open reading frame

More Related Videos

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
13:46

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

6.2K
Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
07:14

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Published on: February 25, 2022

6.4K

Related Experiment Videos

Last Updated: May 16, 2026

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons
10:53

Real-time Imaging of Axonal Transport of Quantum Dot-labeled BDNF in Primary Neurons

Published on: September 15, 2014

15.6K
An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons
13:46

An Improved Protocol to Purify and Directly Mono-Biotinylate Recombinant BDNF in a Tube for Cellular Trafficking Studies in Neurons

Published on: July 11, 2020

6.2K
Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
07:14

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

Published on: February 25, 2022

6.4K

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Genetics

Background:

  • The 5' untranslated region (UTR) of messenger RNA contains regulatory elements that control translation.
  • Upstream open reading frames (uORFs) within the 5' UTR are known regulators of gene expression.
  • Brain-derived neurotrophic factor (BDNF) is crucial for neuronal survival and function, and its expression is tightly regulated.

Purpose of the Study:

  • To investigate the role of uORFs in regulating BDNF translation.
  • To explore methods for modulating BDNF protein levels through uORF manipulation.

Main Methods:

  • Bioinformatic analysis to predict uORFs in BDNF 5' UTRs.
  • Experimental validation of uORF function using reporter constructs.
  • Gene editing techniques (adenine base editing) to disrupt specific uORFs.

Main Results:

  • uORFs were identified in most BDNF transcript isoforms, with several experimentally confirmed to repress translation.
  • Deletion of a 5' UTR exon in a non-expressed BDNF variant led to reporter upregulation, dependent on uORFs and RNA structure.
  • Base editing-mediated disruption of a uORF start codon in a common BDNF variant increased endogenous BDNF protein expression by approximately 1.8-fold.

Conclusions:

  • uORFs significantly contribute to the post-transcriptional regulation of BDNF.
  • Targeting uORFs, particularly via base editing, presents a viable strategy for enhancing BDNF protein expression.