Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct Electrosynthesis of Glycolate From Carbon Dioxide.

Angewandte Chemie (International ed. in English)·2026
Same author

Brain-Derived Neurotrophic Factor and Associated Signaling in Kidney Diseases.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

ATPIF1, the rheostat in mitochondrial bioenergetics and dysfunction in diseases.

Mitochondrion·2026
Same author

Obinutuzumab in Crescentic Anti-Glomerular Basement Membrane Disease with Linear IgG Deposition: A Case Report.

Case reports in nephrology and dialysis·2026
Same author

Klotho suppresses cyst growth in ADPKD through the regulation of ferroptosis and reversing DNA methylation.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2026
Same author

Composition and assembly of the microbial community within the deep chlorophyll maximum layer of the seamount and the adjacent coastal waters in the South China Sea.

mSphere·2026

Related Experiment Video

Updated: Jul 9, 2026

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
10:20

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Published on: August 15, 2012

40.5K

Revisiting Primary Microglia Isolation Protocol: An Improved Method for Microglia Extraction.

Jianwei Li1,2, Zijian Zheng1,2, Menglin Zhang1,2

  • 1Department of Neurosurgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China.

Bio-Protocol
|December 12, 2025
PubMed
Summary
This summary is machine-generated.

This study optimized microglial isolation using a refined shaking method with cytokines. The new protocol yields higher purity and viability, improving efficiency and reducing costs for neuroinflammation research.

Keywords:
Cell cultureMicroglial isolationParkinson’s disease modelPrimary microgliaSNCA A53T transgenic mice

More Related Videos

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
07:54

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

10.3K
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
10:21

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Published on: February 16, 2018

20.1K

Related Experiment Videos

Last Updated: Jul 9, 2026

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
10:20

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Published on: August 15, 2012

40.5K
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
07:54

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Published on: April 13, 2017

10.3K
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
10:21

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Published on: February 16, 2018

20.1K

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Microglia are crucial for central nervous system homeostasis and neurodegenerative disease pathogenesis.
  • Current in vitro microglial isolation methods have limitations in purity, complexity, or cost.
  • Accurate microglial models are needed for studying neuroinflammation and neurodegeneration.

Purpose of the Study:

  • To refine the traditional mixed glial culture shaking method for isolating primary microglia.
  • To enhance microglial viability, proliferation, purity, and yield.
  • To develop a more efficient, cost-effective, and animal-sparing isolation protocol.

Main Methods:

  • Refinement of the classical mixed glial culture shaking technique.
  • Supplementation of specific cytokines during the culture period.
  • Optimization focused on enhancing microglial viability and proliferation.

Main Results:

  • Achieved approximately 90% purity of isolated microglial cells.
  • Maximized the viability and proliferation of primary microglial cultures.
  • Demonstrated increased experimental efficiency with reduced time, animal usage, and cost compared to conventional methods.

Conclusions:

  • The optimized cytokine-supplemented shaking method provides a superior approach for primary microglial isolation.
  • This protocol enhances the quality and quantity of microglia for research applications.
  • The refined method offers a practical and economical solution for generating reliable in vitro models of neuroinflammation and neurodegeneration.