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  1. Home
  3. Root‑preferential Ospub36 Modulates Ros Levels And Regulates Root Development.
  1. Home
  3. Root‑preferential Ospub36 Modulates Ros Levels And Regulates Root Development.

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Root‑preferential OsPUB36 modulates ROS levels and regulates root development.

Sunok Moon1, Yo-Han Yoo2, Ha Eun Park1

  • 1Graduate School of Green Bio-Science & Crop Biotech Institute, Kyung Hee University, Yongin, 17104, Republic of Korea.

Plant Cell Reports
|December 12, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

OsPUB36, a root-preferential E3 ubiquitin ligase, influences rice root development by modulating reactive oxygen species (ROS) homeostasis. Overexpression shortens primary roots and elongates root hairs, impacting plant growth.

Keywords:
OsPUB36ROSRootRoot hairs

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Genetics

Background:

  • Root development is regulated by reactive oxygen species (ROS) gradients.
  • Class III PUB E3 ubiquitin ligases are involved in plant growth.
  • OsPUB36 is a root-preferential E3 ubiquitin ligase in rice.

Purpose of the Study:

  • To investigate the function of OsPUB36 in rice root development.
  • To understand the role of OsPUB36 in reactive oxygen species homeostasis.
  • To explore the potential functional redundancy within the OsPUB31-OsPUB37 gene subclass.

Main Methods:

  • Phylogenetic meta-analysis to identify gene subclasses.
  • Functional characterization of OsPUB36 through overexpression and mutant analysis.
  • RNA-sequencing and proteomic analysis to identify molecular pathways.
  • Histochemical staining to assess hydrogen peroxide levels.

    • Main Results:

    • Overexpression of OsPUB36 in rice led to shortened primary roots and elongated root hairs.
    • OsPUB36 is localized to the endoplasmic reticulum (ER).
    • Upregulation of class III peroxidases and increased hydrogen peroxide (H₂O₂) levels were observed in root hairs of OsPUB36 overexpressing plants.
    • Loss-of-function mutants showed no significant phenotype, indicating functional redundancy.

    Conclusions:

    • OsPUB36 plays a role in regulating rice root development and reactive oxygen species homeostasis.
    • OsPUB36 modulates root hair elongation and primary root length.
    • Functional redundancy exists among class III PUB E3 ubiquitin ligases in rice.