Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Variables Affecting Phosphorescence and Fluorescence01:26

Variables Affecting Phosphorescence and Fluorescence

1.2K
Fluorescence and phosphorescence are essential phenomena in fields like analytical chemistry, biological imaging, and materials science, where they detect molecular properties and visualize cellular structures. Understanding the variables that influence these luminescent behaviors is crucial for maximizing accuracy and efficiency in their applications. These variables can broadly be grouped into chemical structure, solvent properties, and external conditions, each playing a distinct role in...
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cross-Binding of Adenosine by Aptamers Selected Using Theophylline.

Chembiochem : a European journal of chemical biology·2023
Same author

Capture-SELEX for Chloramphenicol Binding Aptamers for Labeled and Label-Free Fluorescence Sensing.

Environment & health (Washington, D.C.)·2023
Same author

Cross-Binding of Four Adenosine/ATP Aptamers to Caffeine, Theophylline, and Other Methylxanthines.

Biochemistry·2023
Same author

Surfactant-Assisted Label-Free Fluorescent Aptamer Biosensors and Binding Assays.

Biosensors·2023

Related Experiment Video

Updated: Jan 8, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.6K

Fluorescent modifications in aptamer switches-positional, structural, and neighboring pair effects on sensor

Albert Zehan Li1, Conrad H T Belzberg1, Mimansa Sidhu1

  • 1Department of Chemistry, University of British Columbia, Vancouver, British Columbia V6T 1Z1, Canada.

Nucleic Acids Research
|December 12, 2025
PubMed
Summary

Modifying aptamer switches with fluorescent reporters can significantly alter their performance. Strategic placement and selection of these reporters are crucial for optimizing biosensor design and function in precision medicine.

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

958
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.1K

Related Experiment Videos

Last Updated: Jan 8, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

15.6K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

958
Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

11.1K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers are nucleic acid ligands used in biosensors for precision medicine.
  • Fluorescent switches are created by conjugating aptamers with reporter molecules.
  • Reporter molecule conjugation can negatively impact aptamer function and biosensor performance.

Purpose of the Study:

  • To evaluate the impact of fluorophore-quencher modifications on aptamer switches.
  • To investigate the interplay between reporter identity, environment, and labeling positions.
  • To understand how these modifications affect aptamer binding properties, kinetics, specificity, and signal resolution.

Main Methods:

  • Utilized a combinatorial array of modifications on aptamer switch sequences.
  • Probed the interplay between reporter identity, local environment, and labeling positions.
  • Analyzed changes in apparent binding affinities and kinetic response rates.

Main Results:

  • Tuned aptamer switch binding properties over a 2-order-of-magnitude range (~230 to 2.3 μM).
  • Adjusted apparent kinetic response rates from ~1000 s to sub-second resolution.
  • Demonstrated that reporter environment and spatial positioning are critical for performance.

Conclusions:

  • Reporter modifications significantly influence aptamer switch performance, including binding affinity, kinetics, specificity, and signal resolution.
  • Strategic design of fluorescent modifications and their placement is essential for optimizing biosensor development.
  • This study provides insights for designing next-generation biosensors with tailored performance for precision medicine.