Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dual Quantum Dot Molecular FRET Probes for Picomolar DNA Hexaplexing.

Small methods·2026

Same author

Cumulative Spectroscopic Detection for Taylor Dispersion Analysis of Nanoparticles.

Analytical chemistry·2026

Same author

Controlled Synthesis of a New Class of Heterostructured Metal Oxides (Cerium, Thorium, Uranium)/Calcium Fluoride Core-Shell Nanocrystals With Atomically Coherent Interfaces.

Angewandte Chemie (International ed. in English)·2026

Same author

Time-Resolved Förster Resonance Energy Transfer Nanoassay Based on CdTe Quantum Dots for Sensitive Detection of Prostate Cancer Antigen 3.

ACS applied nano materials·2026

Same author

FRET Materials for Biosensing and Bioimaging.

Chemical reviews·2025

Same author

Inter-Nanoparticle FRET for Biosensing: Photophysics Versus Size.

Angewandte Chemie (International ed. in English)·2025

Same journal

High-Performance CH-Series Non-Fullerene Acceptors for Organic Photovoltaics.

Accounts of chemical research·2026

Same journal

Design Principles for Negative Thermal Expansion in Two-Dimensional Materials.

Accounts of chemical research·2026

Same journal

Main Group Redox Catalysis: New Frontiers with Germanium and Tin.

Accounts of chemical research·2026

Same journal

Taming Irreversibility in sp<sup>2</sup>-Carbon-Conjugated COFs from Polycrystalline Powders to Single Crystals and Thin Films.

Accounts of chemical research·2026

Same journal

Electroactive Imidazolium Ionic Liquids in Organic Synthesis.

Accounts of chemical research·2026

Same journal

Calix[4]resorcinarene-Based Porous Organic Cages: Synthesis and Applications.

Accounts of chemical research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 1, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

FRET with Upconversion Nanoparticles.

Eduard Madirov¹, Niko Hildebrandt¹

¹McMaster University, Department of Engineering Physics, Hamilton, Ontario M8S 4K1 Canada.

Accounts of Chemical Research

|December 12, 2025

Summary

This summary is machine-generated.

Upconversion nanoparticles (UCNPs) combined with Förster resonance energy transfer (FRET) offer advanced optical biosensing. Optimizing UCNP architecture and surface chemistry is key to overcoming challenges and enabling new bioanalytical applications.

More Related Videos

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

Triplet Fusion Upconversion Nanocapsule Synthesis

Triplet Fusion Upconversion Nanocapsule Synthesis

Published on: September 7, 2022

Related Experiment Videos

Last Updated: Jul 1, 2026

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Published on: November 14, 2015

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications

Published on: November 10, 2017

Triplet Fusion Upconversion Nanocapsule Synthesis

Triplet Fusion Upconversion Nanocapsule Synthesis

Published on: September 7, 2022

Area of Science:

Nanomaterials Science
Biophysics
Optical Spectroscopy

Background:

Upconversion nanoparticles (UCNPs) are valuable for optical biosensing and imaging due to their unique photophysical properties.
Combining UCNPs with Förster resonance energy transfer (FRET) is promising for studying biomolecular interactions.
Challenges exist due to UCNP size, low absorption, and donor-acceptor distance effects on FRET efficiency.

Purpose of the Study:

To review recent advances in UCNP-FRET systems for biosensing and bioimaging.
To discuss strategies for overcoming limitations in UCNP-FRET development.
To highlight future directions for UCNP-FRET applications in biomedicine.

Main Methods:

Development of advanced UCNP core-shell architectures.
Surface functionalization and bioconjugation of UCNPs.
Optimization of FRET acceptor selection and photophysical characterization.
Advanced modeling for understanding UCNP-FRET mechanisms.

Main Results:

Smaller UCNP sizes and novel architectures improve FRET efficiency.
Surface coatings and bioconjugation enhance UCNP-FRET performance.
Understanding donor-acceptor distance and quenching effects is crucial.
Optimized UCNP-FRET systems show potential in biosensing, bioimaging, and theranostics.

Conclusions:

Significant progress has been made in UCNP-FRET technology.
Further research is needed for complete mechanistic understanding and material optimization.
UCNP-FRET holds great promise for translation into clinical bioanalysis and biomedicine.