Related Experiment Video
Updated: Jan 8, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Disruption of HDL antioxidant properties in children and adolescents with obesity
Belén Davico1, Ezequiel Lozano Chiappe1, Laura Gaete2
1Laboratorio de Lípidos y Aterosclerosis, Facultad de Farmacia y Bioquímica, INFIBIOC, Universidad de Buenos Aires, Buenos Aires, Argentina (Davico, Dr Chiappe, Sanchez, Drs Tetzlaff, Ballerini, Rosso, Brites, and Martin); Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina (Davico, Drs Chiappe, Rosso, Brites, and Martin).
Background:
Childhood obesity is associated with alterations in lipoprotein metabolism and increased oxidative stress, assessed by lipid peroxidation products, reactive oxygen species (ROS) and nitric oxide (NO) levels, oxidized/reduced glutathione (GSH/GSSG) ratio, and the activities of superoxide dismutase (SOD) and catalase. High-density lipoproteins (HDL) play an antioxidant role, conditioned by cholesteryl ester transfer protein (CETP), paraoxonase (PON) 1, lecithin:cholesterol acyltransferase (LCAT), lipoprotein-associated phospholipase A2 (Lp-PLA2), and apolipoprotein (apo) A-I.
Objective:
This study aims to evaluate HDL antioxidant capacity in children and adolescents with obesity and the status of its conditioning factors.
Methods:
Thirty children and adolescents, 15 with obesity and 15 normal-weight controls were studied in a cross-sectional observational study. Lipid profile and high-sensitivity C-reactive protein were assessed using standardized methods. Lipid peroxidation products, ROS, NO, GSH and GSSG levels, and catalase, SOD, CETP, LCAT, PON 1 (PON and arylesterase [ARE]) and Lp-PLA2 activities were assessed by developed techniques. Total HDL antioxidant activity and its intrinsic oxidation were evaluated.
Results:
Children with obesity showed lower HDL cholesterol and apo A-I levels (P < .01), reduced CETP (P < .05), ARE (Lp-PLA2 < .05), LCAT (P < .05), and HDL-associated Lp-PLA2 (P < .01) activities, increased HDL intrinsic oxidation (P < 0.01) and reduced total HDL antioxidant activity (P < .05). Patients revealed increased oxidative stress: higher ROS (P < .001) and NO levels (P < .05), lower GSH/GSSG ratio (P < .01) and catalase activity (P < .001).
Conclusions:
Children and adolescents with obesity exhibited reduced HDL antioxidant activity, alterations in its conditioning factors, intrinsic oxidative modification of HDL particles, and increased oxidative stress. These alterations may affect long-term cardiovascular risk in children and adolescents with obesity.
Related Concept Videos
Pharmacokinetics in Obese Patients: Drug Absorption and Distribution
Atherosclerosis III: Management
Obesity
Cholesterol: Significance and Regulation
Considering cholesterol and...
Pharmacokinetics in Obese Patients: Drug Metabolism and Excretion
Lipids: Dietary Sources and Requirements

