Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Robust Functionalized Cotton Fabrics with Zr-Based Tetrahedral Cages for Degrading a Chemical Warfare Agent Simulant.

ACS applied materials & interfaces·2026
Same author

From Molecule to Meaning: Click and Bioorthogonal Chemical Reporters for Plant Systems, Biological Imaging, and Artistic Expression.

Chembiochem : a European journal of chemical biology·2025
Same author

PAMPA: A Software for Peptide Markers and Taxonomic Identification for ZooMS Samples in Archaeology and Paleontology.

Journal of proteome research·2025
Same author

Simultaneous Taxonomic and Sex Identification of <i>Bos</i> and <i>Bison</i> Teeth Using Low-Invasive High-Resolution Mass Spectrometry.

Journal of proteome research·2025
Same author

Classification of Collagens via Peptide Ambiguation, in a Paleoproteomic LC-MS/MS-Based Taxonomic Pipeline.

Journal of proteome research·2025
Same author

Click-ready iridium(iii) complexes as versatile bioimaging probes for bioorthogonal metabolic labeling.

RSC chemical biology·2024

Related Experiment Video

Updated: Jan 8, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.0K

Simultaneous Visualization of Distinct Posttranslational Modification States of β-Catenin Using Genetic Code

Christer Abou Anny1, Sébastien Nouaille2, Isabelle Huvent1

  • 1Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F-59000, Lille, France.

Chembiochem : a European Journal of Chemical Biology
|December 12, 2025
PubMed
Summary

Researchers developed a new method to visualize different posttranslational modification (PTM) states of a single protein in living cells. This technique allows for the study of protein regulation and stability in cancer research.

Keywords:
click chemistrygenetic code expansionposttranslational modificationsprotein imagingβ‐catenin

More Related Videos

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.2K
Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

1.9K

Related Experiment Videos

Last Updated: Jan 8, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.0K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.2K
Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion
13:11

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

1.9K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Posttranslational modifications (PTMs) regulate protein function.
  • Distinct PTMs on a single protein, like β-catenin, influence cellular processes.
  • Understanding PTM interplay is crucial for cancer research.

Purpose of the Study:

  • To develop a novel strategy for simultaneous visualization of distinct PTM states of a single protein.
  • To investigate the functional consequences of different PTMs on β-catenin within living cells.

Main Methods:

  • Genetic code expansion and click chemistry were employed.
  • Site-specific incorporation of unnatural amino acids (phenylselenocysteine) into β-catenin.
  • Engineering of β-catenin fusion proteins with fluorescent reporters (EGFP, mCherry, blue fluorescent protein) to represent different PTM states.
  • Introduction of constructs into Hep3B and HeLa cells via lipofection or TAT-mediated transduction.

Main Results:

  • Successful engineering and cellular delivery of distinct PTM variants of β-catenin.
  • Visualization of unique subcellular localization profiles for each PTM state.
  • Demonstrated enhanced proteasomal degradation resistance for S-GlcNAcylated β-catenin.
  • Established a comparative, cell-based platform for PTM functional probing.

Conclusions:

  • The developed strategy enables simultaneous visualization and functional analysis of distinct PTMs on a single protein.
  • This approach provides insights into the role of PTMs, such as O-GlcNAcylation, in protein stability and cellular regulation.
  • The versatile platform has significant implications for studying protein function in various biological contexts, including cancer.