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Updated: Jan 8, 2026

Quantification of Hypopigmentation Activity In Vitro
Published on: March 6, 2019
The effect of pigment solutions on enhancing tooth bleaching efficacy: A laboratory study
Tutku Baytok Kavci1, Hatice T Yeyen2, Hande Kemaloğlu2
1Department of Restorative Dentistry, Faculty of Dentistry, Ege University, Bornova, Izmir, Turkey, baytoktutku@icloud.com.
Purpose:
To evaluate the effect of various pigments with white or blue coloring properties, which could be applied to bleached enamel surfaces, on the tooth color.
Methods:
Bovine teeth, which exhibit structural characteristics closely resembling human teeth, were used. The samples were subjected to an in-office bleaching procedure using a gel containing 35% hydrogen peroxide. Color measurements were performed using a digital spectrophotometer both before and after bleaching. Subsequently, the following pigments commonly used in cosmetic and medical products were applied to the bleached enamel surfaces: blue covarine, white food dye, titanium dioxide, indigo extract, and nano-hydroxyapatite (n-HAP). Granulated coffee was used as the positive control group, and distilled water served as the negative control. Color changes were analyzed using a spectrophotometer.
Results:
The groups in which blue covarine and titanium dioxide were applied exhibited significantly lighter color values after bleaching compared to the other groups (P< 0.05). While a slight increase in whiteness was observed in the na-HAP group following bleaching, a marked darkening in color occurred in the coffee group.
Clinical Significance:
Light-colored pigments, like blue covarine and titanium dioxide, support color stability after tooth bleaching and may enhance the effectiveness of the bleaching treatment by ensuring longer-lasting esthetic results.
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