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g-C3N4-based complexes for treating in vitro oral cancer cells
Byeong Jin Gu1, So-Young Park2, Wooil Kim3
1Department of Dental Materials, School of Dentistry, Pusan National University, Yangsan, Korea.
Purpose:
To evaluate if gCN (g-C₃N₄) quantum dot (QD) complexes destruct oral cancer cells in vitro in 100 µM H₂O₂ and laser irradiation conditions.
Methods:
gCN complexes were synthesized using polymer, precursor melamine, metal, and photosensitizer. Cell viability tests were performed using cancer and normal cells. To evaluate ROS production by gCN complexes, MB degradation test and RNO-ID assay were performed. Oxygen generation in the test solutions was also evaluated under 100 µM H₂O₂ and laser irradiation conditions.
Results:
Upon the gCN complexes tested, PGC-Ce6 [pvp-(gCN-Cu)-Ce6] destructed over 83% of initial in vitro cancer cells in 100 µM H₂O₂ and laser irradiation conditions, while approximately 88% of normal cells were viable under no laser irradiation. MB solution was greatly degraded (bleached) by PGC-Ce6. Oxygen was generated by PGC-Ce6 in 100 µM H₂O₂ and laser irradiation conditions, yet the amount was little (0.6 mg/L). Absorbance of RNO-ID solution was much decreased with increasing reaction time of PGC-Ce6 in 100 µM H₂O₂ and laser irradiation conditions.
Clinical Significance:
g-C₃N₄ complex (PGC-Ce6) destroyed over 83% of initial in vitro cancer cells in 100 µM H₂O₂ and laser irradiation conditions. The result suggests high potential for clinical tumor treatment of PGC-Ce6 using laser irradiation though further investigation is required.
