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Related Concept Videos

Light Acquisition02:16

Light Acquisition

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In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.
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Updated: May 5, 2026

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
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Spatial transcriptomics reveals expression gradients in developing wheat inflorescences at cellular resolution.

Katie A Long1, Ashleigh Lister2, Maximillian R W Jones1

  • 1John Innes Centre, Norwich Research Park, Norwich NR4 7UH, United Kingdom.

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|December 13, 2025
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Summary
This summary is machine-generated.

Spatial transcriptomics in wheat reveals gene expression patterns shaping inflorescence development. This study maps gene activity at cellular resolution, uncovering factors controlling spikelet formation along the apical-basal axis.

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Area of Science:

  • Plant biology
  • Developmental biology
  • Genomics

Background:

  • Plant inflorescence architecture diversity is driven by gene expression.
  • Wheat spike development involves delayed basal spikelet formation.
  • Previous studies lacked cellular resolution for apical-basal gene expression.

Purpose of the Study:

  • To optimize spatial transcriptomics for wheat inflorescence.
  • To map gene expression at cellular resolution across development.
  • To identify gene expression patterns along the apical-basal axis.

Main Methods:

  • Optimized Multiplexed Error Robust Fluorescence In Situ Hybridization (MERFISH).
  • Analyzed 50,000 cells across 4 developmental stages.
  • Performed cell segmentation and clustering to define expression domains.

Main Results:

  • Identified 18 distinct gene expression domains and markers.
  • Revealed spatio-temporal organization of spikelet and floral development.
  • Characterized spatially coordinated expression patterns in meristems and leaf ridges.

Conclusions:

  • Discovered novel insights into meristem identity and transition factors.
  • Provided a cellular-resolution map of wheat inflorescence development.
  • Made all data publicly available via an interactive WebAtlas interface.