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Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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Updated: Jan 8, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
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Enzyme-based biosensors: emerging tools for advanced biomedical applications.

Edoardo Bocina1, Clarissa Cocuzza1, Chiara Vincenzi1

  • 1Department of Applied Science and Technology, Politecnico di Torino, Corso Duca degli Abruzzi, 24, 10129, Turin, Italy.

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|December 13, 2025
PubMed
Summary
This summary is machine-generated.

Enzyme-based biosensors offer rapid, cost-effective tools for disease monitoring and drug screening. This study highlights their role in cancer and neurodegenerative diseases, showcasing recent innovations and future potential.

Keywords:
Artificial intelligenceDisease detectionDrug screeningEnzyme-based biosensorSelf-powered biosensors

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Area of Science:

  • Biochemistry and Molecular Biology
  • Biotechnology and Bioengineering
  • Medical Diagnostics

Background:

  • Enzymes regulate cell metabolism; their malfunction is linked to pathologies like cancer, neurodegenerative, and inflammatory diseases.
  • Enzyme-based biosensors are crucial for efficient disease monitoring and drug screening.
  • Understanding enzyme roles in disease is key to developing advanced diagnostic tools.

Purpose of the Study:

  • To emphasize the significance of enzymes in cellular processes and disease.
  • To review current enzyme immobilization techniques and detection methods in biosensors.
  • To explore the application of enzyme biosensors in disease detection and drug screening.

Main Methods:

  • Review of enzyme immobilization strategies.
  • Analysis of various enzyme-based detection methodologies.
  • Case studies of enzyme biosensor applications for specific molecule detection.
  • Exploration of drug screening platforms utilizing enzymatic biosensors.

Main Results:

  • Demonstrated the critical role of enzymes in cancer and neurodegenerative disease progression.
  • Presented recent advancements in enzyme immobilization and detection technologies.
  • Showcased the versatility of enzyme biosensors in diagnostics and drug discovery.

Conclusions:

  • Enzyme biosensors are versatile tools with significant potential in healthcare.
  • Future developments include integration with artificial intelligence and self-powered devices.
  • Enzymatic biosensors represent a cutting-edge technology for novel applications.