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Transcriptomes of thirteen healthy feline tissue types.

Jessica J Hayward1,2, Susan Garrison3, Isabel Hernandez3

  • 1Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA. jessica.hayward@cornell.edu.

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|December 13, 2025
PubMed
Summary

Researchers created a valuable transcriptome dataset from thirteen healthy feline tissue types. This resource aids in understanding feline genetic diseases and improving the domestic cat genome annotation.

Keywords:
Felis catusDomestic catHealthy control tissueRNA-seqTranscriptomemRNA enrichmentrRNA depletion

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Area of Science:

  • Genomics
  • Comparative Medicine
  • Feline Biology

Background:

  • Domestic cats (Felis catus) are susceptible to genetic diseases, serving as models for human conditions.
  • Access to healthy feline tissue for genetic studies is limited.
  • Thirteen healthy feline tissue types were selected based on common disease prevalence.

Purpose of the Study:

  • To generate a comprehensive transcriptome dataset from healthy feline tissues.
  • To provide a foundational resource for feline genetic disease research.
  • To enhance the annotation of the domestic cat genome.

Main Methods:

  • Transcriptome sequencing of 78 samples from six healthy cats.
  • Utilized both mRNA-enriched and rRNA-depleted library preparation methods.
  • Ensured data quality through Principal Components Analysis and comparison with human tissue expression data.

Main Results:

  • High-quality transcriptome datasets were generated.
  • Principal Components Analysis confirmed sample clustering by tissue type.
  • Demonstrated high levels of expected tissue-specific gene expression and pathway enrichment.

Conclusions:

  • The feline transcriptome dataset is publicly available for future research.
  • Facilitates functional studies for feline complex diseases and genome annotation.
  • Enables exploration of gene expression in disease-associated variants and environmental interactions.