Related Experiment Video For Catalytic hairpin assembly
Updated: Jan 8, 2026
Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform
Published on: November 30, 2016
A spatially-programmed DNA nanoplatform for multiplexed and orthogonal miRNA profiling enables robust diagnosis of
Lingjie Ma1, Ningfang Wu1, Aiduo Hou1
1Key Laboratory of Laboratory Medicine, Ministry of Education, Zhejiang Provincial Key Laboratory of Medical Genetics, Cixi Biomedical Research Institute, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, 325035, PR China.
Abstract:
Sensitive and simultaneous detection of a panel of microRNAs (miRNAs) is crucial for the precise early diagnosis of colorectal cancer (CRC) in complex environments and clinical samples. Here, we developed a CRC screening platform based on a multi-branched DNA scaffold assembled with multiple fluorophore-modified DNA hairpins for the simultaneous analysis of three CRC-associated miRNAs (miR-31, miR-576, and miR-4669). The spatially-programmed DNA nanoplatform provided an ideal framework for the precise organization of DNA hairpins at the nanoscale, which function as both target recognition elements and independent catalytic hairpin assembly (CHA) modules. This design enabled simultaneous miRNA sensing without cross-talk, accelerated reaction kinetics and enhanced, improved resistance to enzymatic degradation. The sensing platform exhibited a strong linear response across both low and high concentration ranges for all three miRNAs, achieving detection limits as low as 1.0 fM. The excellent performance of the sensing platform in diverse environments, including simulated samples, and real clinical specimens, further demonstrated its strong potential for early CRC screening.
