Rapid analysis of ginsenosides in biological samples using an efficient embedded dispersive solid-phase...

Area of Science:

• Analytical Chemistry
• Pharmacognosy
• Materials Science

Background:

• Ginsenosides are key bioactive compounds in Panax ginseng.
• Accurate and rapid analysis of ginsenosides in biological matrices is crucial for research and quality control.
• Existing methods may be time-consuming or require complex sample preparation.

Purpose of the Study:

• To develop a new, rapid, and efficient method for analyzing ginsenosides in biological samples.
• To utilize novel lecithin@silica particles for sample preparation.
• To validate the method's accuracy, recovery, and matrix effects.

Main Methods:

• Preparation of lecithin@silica particles by embedding SiO2 with lecithin.
• Optimization of embedded dispersive solid-phase microextraction (E-DSPE) conditions: particle mass, absorption time, sample pH, and elution solvent.
• Analysis of four ginsenosides (Rg1, Re, Rb1, Rd) in enzymatic solutions, decoction, and urine samples.

Main Results:

• Optimized E-DSPE conditions achieved high recovery rates (81.94–116.25%) and low relative standard deviation (<2.91%).
• The method demonstrated acceptable matrix effects (-11.3% to 18.21%) across different biological samples.
• Lecithin@silica particles proved effective for ginsenoside extraction.

Conclusions:

• The developed E-DSPE method offers a rapid, green, cost-effective, and efficient approach for ginsenoside analysis.
• This technique is suitable for the quantitative determination of ginsenosides in complex biological matrices.
• The lecithin@silica material shows promise for microextraction applications.