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Related Experiment Video

Updated: Jan 8, 2026

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

Published on: October 15, 2013

IL-27 effects on HIV_Gag-specific CD4 and CD8 T cell function.

Maryam Abdussamad¹, Grace Katz¹, Jie Cheng¹

¹Department of Microbiology and Immunology, Georgetown University School of Medicine, 3970 Reservoir Road, N.W, New Research Building, Room EG19A, Washington, DC 20057, USA.

Frontiers in Virology (Lausanne, Switzerland)

|December 15, 2025

Summary

Interleukin-27 (IL-27) may improve immune responses in people with HIV (PWH). This study found IL-27 enhances HIV-specific CD8 T cell function, potentially improving outcomes for PWH.

Keywords:

HIV HIV-specific T cells IL-27 effects on T cells IL27 T cell immune activation

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In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

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Published on: October 15, 2013

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New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

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Published on: May 30, 2013

Area of Science:

Immunology
Virology
Cellular Biology

Background:

Persistent T cell activation and inflammation increase morbidity and mortality in people with HIV (PWH) despite suppressed viral replication.
CD8 T cells in PWH often exhibit dysfunction and express checkpoint receptors.
Interleukin-27 (IL-27), a cytokine with known antiviral properties, has an unclear role in HIV-specific T cell function.

Purpose of the Study:

To investigate the effects of IL-27 on HIV-specific T cells.
To determine if IL-27 can enhance the function of HIV-specific T cells in the context of chronic HIV infection.

Main Methods:

Evaluated IL-27's impact on T cell function by measuring cytokine secretion, proliferation, and cytotoxicity.
Utilized unbiased clustering analysis to examine differential effects on distinct T cell populations.

Main Results:

IL-27 was found to upregulate cytokine secretion and cytotoxic potential in HIV-specific CD8 T cells.
IL-27 promoted the trafficking of proliferating HIV-specific CD8 T cells expressing checkpoint receptors TIGIT and PD-1.
Unbiased clustering suggested IL-27 may have varied effects on different HIV-specific T cell subsets.

Conclusions:

IL-27 demonstrates potential to enhance T cell function in chronic HIV infection.
These findings suggest IL-27 as a possible therapeutic agent to bolster immune responses against HIV.