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Updated: Jan 8, 2026
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An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
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Genome sequence of bacteriophage AmiCi24 isolated using Arthrobacter globiformis
Paige Baldwin1, Michael Bennett1, Evangelia Buonamici1
1Department of Biological and Biomedical Sciences, College of Science and Mathematics, Rowan University, Glassboro, NJ 08028, USA.
Micropublication Biology
|December 15, 2025
Summary
A novel siphoviral bacteriophage, AmiCi24, was discovered in New Jersey soil. This temperate phage infects Arthrobacter globiformis and has a genome encoding 68 genes.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Bacteriophages are viruses that infect bacteria and play crucial roles in microbial ecosystems.
- Actinobacteriophages are bacteriophages that specifically infect Actinobacteria, a diverse and important group of bacteria.
- Characterizing novel bacteriophages aids in understanding phage diversity and their potential applications.
Purpose of the Study:
- To isolate and characterize a novel siphoviral bacteriophage from a soil environment.
- To determine the genomic features of the newly discovered bacteriophage.
- To classify the bacteriophage based on its genetic content and host range.
Main Methods:
- Isolation of bacteriophage from a soil sample using Arthrobacter globiformis as the host.
- Genomic DNA extraction and sequencing.
- Bioinformatic analysis of the bacteriophage genome to predict protein-coding genes and phylogenetic placement.
Main Results:
- A novel siphoviral bacteriophage, designated AmiCi24, was successfully isolated.
- AmiCi24 possesses a genome of 38,466 base pairs encoding 68 predicted protein-coding genes.
- Phylogenetic analysis based on gene content assigned AmiCi24 to actinobacteriophage cluster AS, subcluster AS3.
Conclusions:
- AmiCi24 represents a new addition to the known diversity of siphoviral bacteriophages infecting Actinobacteria.
- The genomic data suggests AmiCi24 is a temperate bacteriophage.
- Further studies on AmiCi24 could elucidate its biological properties and potential use in phage therapy or other applications.
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