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Anterior Cruciate Ligament Bundles Elongation Patterns During Fast Running and Single-Leg Drop Landing: An In Vivo
Kohei Kamada1,2, Tom Gale2, Koji Nukuto1,2,3
1Biodynamics Laboratory, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Orthopaedic Journal of Sports Medicine
|December 15, 2025
Summary
The anterior cruciate ligament's anteromedial bundle elongates more than the posterolateral bundle during fast running. During single-leg drop landings, both bundles elongate similarly, informing ACL graft tensioning strategies.
Area of Science:
- Orthopedic Surgery
- Biomechanics
- Sports Medicine
Background:
- Understanding anterior cruciate ligament (ACL) bundle elongation is crucial for optimizing graft tensioning in double-bundle ACL reconstructions.
- Limited in vivo data exists on ACL anteromedial bundle (AMB) and posterolateral bundle (PLB) elongation during high-impact activities.
Purpose of the Study:
- To quantify the relative elongation of the AMB and PLB during fast running and single-leg drop landing.
- To determine the knee flexion angle at which peak elongation occurs for each bundle.
Main Methods:
- Nineteen healthy athletes underwent biplane radiography during fast running and single-leg drop landing.
- A validated model-based tracking process analyzed tibiofemoral motion using subject-specific 3D bone models.
- Relative elongation of AMB and PLB was calculated and compared to supine CT measurements.
Main Results:
- During fast running, AMB peak relative elongation was significantly greater than PLB (2.6% difference).
- During single-leg drop landing, PLB and AMB peak relative elongation were comparable (-0.9% difference).
- Peak elongation occurred at different knee flexion angles for each activity and bundle.
Conclusions:
- The AMB exhibits greater elongation than the PLB during fast running.
- The PLB and AMB show similar elongation patterns during single-leg drop landing.
- Findings suggest specific graft fixation angles may better replicate native bundle function during high-impact activities.

