Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.0K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.0K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

8.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.9K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

16.5K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
16.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The shape of ribbons: from polymers to surfaces.

Soft matter·2026
Same author

The shape of cleaved tethered membranes.

Soft matter·2025
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.6K

Phase behavior of active particle-loaded vesicles.

A D Chen1, A Cacciuto1

  • 1Department of Chemistry, Columbia University, 3000 Broadway, New York, New York 10027, USA.

The Journal of Chemical Physics
|December 15, 2025
PubMed
Summary

Flexible vesicles with active particles show complex phase behavior, forming liquid crystal-like structures. Particle activity can destabilize or create novel ordered arrangements within the vesicles.

More Related Videos

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

4.0K
In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)
06:34

In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)

Published on: June 20, 2025

1.7K

Related Experiment Videos

Last Updated: Jan 8, 2026

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
10:08

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy

Published on: October 24, 2017

9.6K
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

4.0K
In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)
06:34

In vitro Reconstitution of Cytoskeletal Networks inside Phase Separated Giant Unilamellar Vesicles (GUVs)

Published on: June 20, 2025

1.7K

Area of Science:

  • Soft matter physics
  • Complex fluids
  • Biophysics

Background:

  • Vesicles are fundamental structures in biology and soft matter.
  • Active particles introduce self-propulsion and emergent behaviors in confined systems.
  • Understanding phase behavior is crucial for designing active matter systems.

Purpose of the Study:

  • To investigate the phase behavior of flexible 2D vesicles containing self-avoiding active particles.
  • To construct phase diagrams based on system density, particle loading, and particle activity.
  • To analyze the stability and formation of structures within these active vesicles.

Main Methods:

  • Computational modeling of flexible two-dimensional vesicles.
  • Inclusion of self-avoiding active particles with tunable activity levels.
  • Construction and analysis of structural phase diagrams.

Main Results:

  • Identification of non-trivial, liquid crystal-like structures within the vesicles.
  • Demonstration that particle activity can destabilize ordered phases by exciting soft modes.
  • Observation of novel ordered configurations induced by particle activity, not present in passive systems.

Conclusions:

  • The phase behavior of active particle-loaded vesicles is rich and tunable.
  • Particle activity plays a dual role, capable of both disrupting and creating order.
  • These findings offer insights into the design principles for active soft matter systems.