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Updated: Jan 8, 2026
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
Published on: August 4, 2016
Investigating the role of inflammatory bowel disease-associated gene expression in oral cancer using single-cell RNA
Yongwei Cheng1, Zhenyin Liu2, Shifeng Xie2
1Key Laboratory for Precision Diagnosis and Treatment of Pediatric Digestive System Diseases, Endoscopy Center and Gastroenterology Department, Shenzhen Children's Hospital, Shenzhen, 518036, Guangdong Province, China.
Purpose:
This study aimed to investigate the expression of infl ammatory bowel disease (IBD)-associated genes in oral cancer and to elucidate the cellular and molecular pathways that may serve as potential therapeutic targets.
Methods/Patients:
Oral cancer tissue samples were subjected to single-cell RNA sequencing to characterize cell clusters and gene expression patterns. UMAP and t-SNE were used for dimensionality reduction and visualization of cellular subgroups. Dot plots were applied to identify key gene expression signatures. Gene Set Enrichment Analysis (GSEA) was performed to examine pathways associated with NFKBIA expression.
Results:
Single-cell analysis revealed heterogeneous cell populations, including T cells, fi broblasts, and malignant cells. Diff erential expression analysis identifi ed elevated levels of LYZ and IL7R in specifi c cell subsets. In several clusters, IBD-related genes such as NFKBIA, RB1CC1, and ATG5 were upregulated. GSEA indicated that NFKBIA expression was signifi cantly associated with the chemokine-mediatedsignaling pathway, a process implicated in tumor growth.
Conclusions:
This study highlights the cellular heterogeneity and distinct gene expression programs inoral cancer, emphasizing the relevance of IBD-associated genes. The association between NFKBIA expression and chemokine-mediated signaling provides insights that may contribute to the development of personalized therapeutic strategies for IBD-related mechanisms in oral cancer.
