Related Experiment Video
Updated: Jan 8, 2026
Cardiac Blood Collection via Subclavian Puncture in Mice
Published on: November 28, 2025
Cardiac Blood Collection via Subclavian Puncture in Mice
Siyi Zhong1, Qingya Dang2, Yuting Ma2
1College of Agriculture, Yanbian University.
Insights
Collecting large volumes of mouse blood is challenging. Subclavian cardiac puncture offers a simple, effective method for high-quality blood samples, recovering ~50% of total blood volume with reduced contamination.
Area of Science:
- * Animal research methodology
- * Experimental physiology
- * Biomedical research techniques
Background:
- * Mouse blood collection is crucial for experimental studies.
- * Existing methods for large-volume blood collection, such as large-vessel or cardiac puncture, present challenges.
- * Large-vessel collection often requires invasive surgery, while cardiac puncture methods vary in technique and efficacy.
Purpose of the Study:
- * To evaluate the subclavian cardiac puncture technique for mouse blood collection.
- * To assess the ease of operation and sample quality of this method.
- * To determine the recovery volume and contamination levels compared to other techniques.
Main Methods:
- * Detailed examination of the subclavian cardiac puncture technique.
- * Comparison of puncture sites and procedural advantages/disadvantages.
- * Validation of blood recovery percentage and tissue fluid contamination levels.
Main Results:
- * The subclavian cardiac puncture method is easy to operate and yields high-quality samples.
- * This technique recovers approximately 50% of the total mouse blood volume.
- * Significantly reduced tissue fluid contamination was observed with this method.
Conclusions:
- * Subclavian cardiac puncture is a viable and efficient method for large-volume mouse blood collection.
- * The technique's simplicity and sample quality make it suitable for various research fields.
- * Applicable to immunology, toxicology, and metabolic studies, enhancing experimental reliability.
Abstract:
In experimental studies, mouse blood is a common experimental sample. However, there are still some challenges for the collection of large volumes of blood. The common methods of terminal large-volume blood collection include large-vessel blood collection and cardiac puncture collection. Large-vessel blood collection often requires surgery and is difficult to perform. In contrast, cardiac puncture is simple and easy to operate. However, there are also different methods for blood sampling by cardiac puncture. Different methods have different puncture sites, and each has its advantages and disadvantages. In this study, we provided a detailed examination of the subclavian cardiac puncture technique for blood collection, emphasizing its ease of operation and its ability to yield high-quality samples. This study validates that the subclavian cardiac puncture blood collection method can recover approximately 50% of the total blood volume and significantly reduce tissue fluid contamination. The method can be applied to multiple research disciplines, including immunology, toxicology, and metabolic studies.
