Simple and Effective Procedure for Hemostasis in Mouse Arteries

Area of Science:

• Biomedical Engineering
• Surgical Innovation
• Animal Models

Background:

• Arterial puncture in mice often requires ligation due to bleeding, causing ischemic injury and compromising research.
• Existing hemostasis methods can lead to complications, impacting the reliability of animal models.

Purpose of the Study:

• To introduce and evaluate a novel fat-wrapping technique for arterial puncture hemostasis in mice.
• To prevent postoperative ischemia and secondary complications in animal models.

Main Methods:

• Harvesting autologous adipose tissue fragments from mice.
• Performing arterial puncture and wrapping the site with adipose tissue for hemostasis.
• Assessing cerebral blood flow using Laser Speckle Contrast Imaging (LSCI) and performing histological analysis (H&E, Nissl staining).

Main Results:

• The fat-wrapping technique achieved reliable hemostasis without compromising arterial patency.
• LSCI showed no significant differences in cerebral blood flow between sham and operated groups.
• Histological analysis confirmed preserved brain architecture and neuronal integrity at 7, 14, and 28 days post-surgery.

Conclusions:

• Autologous fat-wrapping is a simple, effective method for arterial hemostasis in mice.
• This technique prevents ischemic complications, enhancing the validity of animal research.
• The method shows significant translational potential for clinical applications.