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Updated: Jan 8, 2026

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Lateral-scanning through-focus microscopy for high-speed three-dimensional localization of subsurface defects
This study introduces a novel lateral-scanning through-focus microscopy for precise 3D defect localization in transparent materials. The innovative technique achieves sub-pixel accuracy, enhancing quality control in manufacturing and inspection.
Area of Science:
- Optical Metrology
- Materials Science
- Quality Control
Background:
- Accurate 3D localization of subsurface defects is vital for manufacturing and inspection.
- Traditional optical methods face limitations in achieving both large field of view and high localization accuracy.
Purpose of the Study:
- To develop an advanced microscopy technique for precise 3D localization and detection of subsurface defects.
- To overcome the limitations of conventional optical measurement techniques in transparent samples.
Main Methods:
- Proposed a lateral-scanning through-focus microscopy system.
- Utilized optical axis tilting to capture defects at varying depths within a single image.
- Integrated horizontal scanning for extended field of view 3D localization.
Main Results:
- Achieved sub-pixel accuracy in 3D defect localization using ring light illumination on SiO2 samples.
- Demonstrated a relative standard deviation of 0.77% for depth measurements.
- Validated wide-field 3D localization capability with microsphere samples.
Conclusions:
- The proposed lateral-scanning through-focus microscopy enables accurate wide-field 3D localization of subsurface defects.
- Ring light illumination significantly enhances localization accuracy and precision.
- The system offers a promising solution for non-destructive testing in transparent materials.
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