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Related Experiment Video

Updated: Jan 8, 2026

Design and Implementation of an Automated Illuminating, Culturing, and Sampling System for Microbial Optogenetic Applications
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Single copy optogenetic system for Streptomyces.

Airi Watanabe1, Ryuta Noya1, Rio Yamada1

  • 1Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, 252-0880, Japan.

Scientific Reports
|December 15, 2025
PubMed
Summary

Scientists developed a new light-inducible gene expression system (iLiEX) for Streptomyces bacteria. This system enables precise control over protein production using blue-green light, offering significant advantages over existing methods.

Keywords:
Strepomyces griseusExpression systemLitROptogenetics

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • LitR is a blue-green light-sensing transcriptional regulator utilizing coenzyme B12.
  • Streptomyces species are important Gram-positive bacteria, with S. griseus known for streptomycin production.

Purpose of the Study:

  • To develop a genome-integrative, light-inducible expression (iLiEX) system in Streptomyces griseus.
  • To enable precise, light-controlled overproduction of proteins in Streptomyces.

Main Methods:

  • Integration of LitR, T7 RNA polymerase, and serine integrase into the Streptomyces genome.
  • Optimization of the T7 RNA polymerase ribosome-binding site and introduction of T7 lysozyme.
  • Modification of the T7 promoter length for enhanced versatility.

Main Results:

  • Achieved light-dependent overproduction of catechol-2,3-dioxygenase and GUS, with GUS activity 39-fold higher than the ermE* promoter.
  • Demonstrated functionality across multiple Streptomyces species (S. coelicolor, S. lividans, S. albus, S. avermitilis).
  • Successfully visualized light-induced overexpression of fluorescent proteins, chromogenic proteins, and pigment synthesis enzymes.

Conclusions:

  • The developed iLiEX system is a versatile, single-copy, light-inducible overexpression tool for Streptomyces.
  • The system allows for high-level production of various proteins, including secreted enzymes like laccase and transglutaminase.
  • iLiEX offers broad applicability across diverse Streptomyces species for controlled gene expression.