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Plasmonic Metal-Phenolic Network Nanoprobes for Multiplex Dual-Mode Immunophenotyping.

Lara González-Cabaleiro1,2, Zhixing Lin3,4, Lorena Vázquez-Iglesias1

  • 1CINBIO, Universidade de Vigo, Campus Universitario As Lagoas, Marcosende, 36310 Vigo, Spain.

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Summary

Researchers developed dual-mode nanoprobes for precise cell identification. This metal-phenolic network (MPN) technology enhances multiplexed immunophenotyping accuracy and reduces errors in cell analysis.

Keywords:
SERS tagscell targetingdual modemetal−phenolic network

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Cell Biology

Background:

  • Accurate multiplexed immunophenotyping is crucial for dissecting cellular heterogeneity.
  • Dual-mode detection strategies, combining surface-enhanced Raman scattering (SERS) and fluorescence, offer enhanced accuracy and cross-validation to minimize false positives.

Purpose of the Study:

  • To develop novel dual-mode nanoprobes for precise and scalable immunophenotyping.
  • To integrate Raman reporters and fluorescent antibodies within a single probe for simultaneous detection.

Main Methods:

  • Fabrication of dual-mode nanoprobes using metal-phenolic networks (MPNs) on hollow plasmonic nanocapsules.
  • One-step conjugation of biorecognition elements via metal-phenolic coordination.
  • Multiplex detection of epidermal growth factor receptor (EGFR) and CD44 using SERS and flow cytometry.

Main Results:

  • Demonstrated successful multiplexed detection of EGFR and CD44 in cultured cells.
  • Accurately determined cell population distributions (∼63% HER14, ∼37% HEK-293) using SERS, corroborated by flow cytometry.
  • Validated the efficacy of MPN nanoprobes for dual-mode detection.

Conclusions:

  • Plasmonic MPN nanoprobes offer a robust and scalable platform for accurate immunophenotyping.
  • This dual-mode approach enhances cell analysis and holds potential for targeted cell imaging and diagnostics.