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MYC2-LNC86472-miR166a-HB15 Module Mediates the Effect of Jasmonic Acid on Soft Rot Resistance in Colored Calla Lily
Yi Wang1, Tuo Yang2, Lele Wu1,3
1Institute of Grassland, Flowers and Ecology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Plant, Cell & Environment
|December 16, 2025
Summary
Soft rot disease in calla lilies involves long noncoding RNAs (lncRNAs). Jasmonic acid (JA) downregulates LNC86472, releasing miR166a to combat the Pectobacterium carotovorum pathogen.
Area of Science:
- Plant Pathology
- Molecular Biology
- Biochemistry
Background:
- Soft rot, caused by Pectobacterium carotovorum, significantly impacts colored calla lily production.
- Understanding the molecular mechanisms of plant defense against soft rot is crucial for disease management.
Purpose of the Study:
- To investigate the role of long noncoding RNAs (lncRNAs) in the colored calla lily's response to Pectobacterium carotovorum infection.
- To elucidate the regulatory pathway involving lncRNAs, microRNAs, and plant defense genes.
Main Methods:
- Transcriptome sequencing of infected and control calla lily leaves to identify lncRNAs.
- Differential gene expression analysis to detect changes in lncRNA and mRNA levels post-infection.
- Functional validation using transient expression and gene silencing techniques.
- Analysis of hormone levels (jasmonic acid) and transcription factor binding (MYC2).
Main Results:
- 35,175 potential lncRNAs were identified, with many showing differential expression upon infection.
- LNC86472 was identified as a key lncRNA acting as an endogenous target mimic for miR166a.
- miR166a directly targets HB15 transcripts, which are involved in activating immune responses.
- Overexpression of LNC86472 or HB15 compromised plant immunity, while silencing enhanced resistance.
- Jasmonic acid (JA) signaling pathway mediates the downregulation of LNC86472 via MYC2 binding.
Conclusions:
- A novel regulatory pathway is proposed where JA-induced LNC86472 downregulation releases miR166a, leading to HB15 transcript cleavage and enhanced plant immunity.
- This study reveals the critical role of lncRNAs, specifically LNC86472, in the molecular defense against soft rot in colored calla lilies.
- Findings offer new targets for developing strategies to improve resistance against Pectobacterium carotovorum.
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