Metal polyphenol CA-V nanozymes promote acute wound healing by destroying the ROS-inflammation cascade cycle

Area of Science:

• Biomaterials Science
• Nanotechnology
• Wound Healing Research

Background:

• Skin ulceration severely impairs barrier function, delaying healing due to high reactive oxygen species (ROS) and inflammatory factors.
• Current clinical treatments for skin ulcers have limited efficacy, necessitating novel therapeutic strategies.
• Targeting the ROS-inflammatory cycle is crucial for accelerating early wound healing.

Purpose of the Study:

• To construct a metal polyphenol nanozyme with multienzyme activity to address ROS-induced inflammation and promote wound healing.
• To investigate the therapeutic potential of caffeic acid-vanadium (CA-V) nanoparticles for acute wound treatment.

Main Methods:

• Hydrothermal synthesis of caffeic acid and ammonium metavanadate, followed by purification to obtain CA-V nanoparticles.
• In vitro assays to evaluate antioxidant (SOD-like, CAT-like) and ROS/RNS scavenging activities, and fibroblast migration.
• In vivo studies in mice, including oxidative stress models and RNA sequencing, to assess anti-inflammatory, pro-angiogenic, and wound healing effects.

Main Results:

• CA-V nanoparticles demonstrated significant SOD-like and CAT-like activities, effectively eliminating ROS/RNS and promoting fibroblast migration.
• In vitro and in vivo experiments showed CA-V possesses strong antioxidant and antiapoptotic properties, downregulating key inflammatory pathways (TNF-α, IL-17).
• CA-V accelerated wound healing in mice by reducing inflammation, promoting angiogenesis, and improving collagen arrangement, with good biosafety.

Conclusions:

• The developed CA-V nanozyme effectively targets the ROS-inflammatory cycle, offering a promising therapeutic strategy for accelerating early acute wound healing.
• CA-V nanozymes exhibit potent antioxidant, anti-inflammatory, and pro-healing properties, making them a viable option for advanced wound care.
• This approach provides a novel and effective therapeutic strategy for promoting early wound healing by mitigating oxidative stress and inflammation.