Related Experiment Video

Updated: Jan 8, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

2.8K

Genomic Characterization of Intestinal Colonizing Pseudomonas juntendi Strains Harboring bla VIM-2

Weidong Zhu1, Junli Zhang2, Ruishan Liu3

  • 1Zhuji Affiliated Hospital of Wenzhou Medical University, Zhuji, Zhejiang Province, People's Republic of China.

Infection and Drug Resistance
|December 16, 2025
PubMed
Abstract

Insights

Genomic analysis revealed two carbapenem-resistant Pseudomonas juntendi strains from human feces carrying blaVIM-2. These strains are clonally related and linked to an animal isolate, highlighting potential human-animal resistance gene flow.

Area of Science:

  • Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • Pseudomonas juntendi is an emerging pathogen.
  • Carbapenem resistance poses a significant public health threat.
  • Understanding the genomic basis of resistance is crucial for control.

Purpose of the Study:

  • To characterize the genome of carbapenemase-producing P. juntendi harboring blaVIM-2.
  • To elucidate mechanisms of carbapenem resistance.
  • To evaluate mobile genetic element-mediated dissemination.

Main Methods:

  • Hybrid whole-genome sequencing (Oxford Nanopore and Illumina).
  • Comparative pangenomics and core genome phylogeny.
  • S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) for plasmid analysis.

Main Results:

  • Two clonally related P. juntendi strains (L2353hy/L2891hy) from human feces identified.
  • Strains harbored 13 antimicrobial resistance genes, including blaVIM-2, blaOXA-246, and tet(A).
  • Human isolates showed close phylogenetic links to an animal-derived P. juntendi strain.

Conclusions:

  • This study expands genomic understanding of carbapenem-resistant P. juntendi.
  • Bidirectional resistance gene flow at the human-animal interface is suggested.
  • A One Health approach is recommended for surveillance.
Keywords:
Pseudomonas juntendiantimicrobial resistanceblaVIM-2whole-genome sequencing

More Related Videos

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

1.0K
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

7.6K

Related Concept Videos

Bacterial Flora of the Large Intestine01:29

Bacterial Flora of the Large Intestine

The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
1.5K
Antibiotic Selection00:57

Antibiotic Selection

Overview
59.3K
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1.1K
Bacterial Phylum Verrucomicrobiota01:26

Bacterial Phylum Verrucomicrobiota

The phylum Verrucomicrobiota comprises at least four characterized orders, with most species classified within the order Verrucomicrobiotales. Members of this phylum are either aerobic or facultatively aerobic, with the ability to ferment sugars. A notable exception is the genus Methylacidiphilum, which consists of aerobic methanotrophs. Additionally, some Verrucomicrobiota establish symbiotic relationships with protists. These bacteria are widely distributed across various environments,...
490
Urinary Tract Infection II: Pathophysiology01:25

Urinary Tract Infection II: Pathophysiology

The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
530
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
463