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Related Concept Videos

Bioplastics01:27

Bioplastics

Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...

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Preparation of Tunable Extracellular Matrix Microenvironments to Evaluate Schwann Cell Phenotype Specification
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Nanoplastics in Duckweed: Single-Cell Responses and Recovery.

Wenke Yuan1,2,3, Elvis Genbo Xu4, Dong Zhu5

  • 1Hubei Key Laboratory of Wetland Evolution & Ecological Restoration, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China.

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Summary

Duckweed exposed to nanoplastics showed reduced growth and partial recovery, with cell-specific molecular responses identified. This study reveals how aquatic plants cope with nanoplastic contamination at a cellular level.

Keywords:
aquatic plantsmolecular responsenanotracer analysisplastic contaminationsingle-nucleus RNA sequencingtranscriptomics

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Area of Science:

  • Environmental Science
  • Ecotoxicology
  • Molecular Biology

Background:

  • Micro- and nanoplastics are pervasive aquatic contaminants.
  • Nanoplastics pose risks to aquatic organisms and ecosystems.
  • Cell-specific responses of plants to nanoplastics are poorly understood.

Purpose of the Study:

  • Investigate duckweed's response and recovery to nanoplastics.
  • Elucidate cell-specific molecular mechanisms.
  • Understand nanoplastic interactions with aquatic plants.

Main Methods:

  • Single-nucleus RNA sequencing (scRNA-seq).
  • Enzymatic assays.
  • Europium-doped nanoplastic tracing.

Main Results:

  • Nanoplastics reduced duckweed reproduction and root length via oxidative damage.
  • scRNA-seq identified cell-type-specific responses in mesophyll, mestome sheath, epidermis, and parenchyma.
  • Recovery phase showed increased gene expression in carbon metabolism, membrane transport, and stress pathways.
  • Nanotracer studies confirmed root/frond absorption and partial excretion.

Conclusions:

  • Duckweed exhibits cell-specific molecular strategies to cope with nanoplastic stress.
  • Partial recovery involves significant molecular pathway adjustments.
  • This research provides mechanistic insights into plant-nanoplastic interactions.