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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

Updated: May 8, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

Multifunctional DNA Nanonets for a Novel Self-Powered Biosensor.

Suping Deng^1,2, Futing Wang², Yujin Li²

¹Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China.

|December 16, 2025

Summary

This summary is machine-generated.

This study presents a novel self-powered biosensor for detecting tobramycin (TOB) using DNA nanonets and a bimetallic metal-organic framework. The developed biosensor achieves ultrasensitive detection of TOB with high accuracy and real-time monitoring capabilities.

Keywords:

DNA nanonets metal organic frameworks nanozymes self-powered biosensor tobramycin

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Area of Science:

Biomedical Engineering
Nanotechnology
Analytical Chemistry

Background:

Tobramycin (TOB) is an antibiotic requiring sensitive detection methods.
Existing biosensors often lack self-powering capabilities or sufficient sensitivity.
Metal-organic frameworks offer unique catalytic and conductive properties for biosensing.

Purpose of the Study:

To develop an ultrasensitive, self-powered biosensor for tobramycin detection.
To utilize multifunctional DNA nanonets and a bimetallic metal-organic framework (Fe/Co-MIL-88(NH2)) for enhanced performance.
To enable real-time, smartphone-based monitoring of tobramycin levels.

Main Methods:

Fabrication of a self-powered biosensor incorporating a Fe/Co-MIL-88(NH2) bimetallic metal-organic framework in the bioanode and gold nanoparticle-decorated carbon nanotubes in the biocathode.
Utilizing DNA nanonets to adsorb [Ru(NH3)6]3+ for signal amplification and open-circuit voltage generation.
Employing a capacitor for current amplification and a smartphone with Bluetooth for real-time data readout.

Main Results:

The biosensor demonstrated ultrasensitive detection of tobramycin with a limit of detection as low as 0.41 fM.
Achieved a wide detection range from 1.0 to 10^7 fM.
Exhibited excellent selectivity, stability, reproducibility, and applicability in actual samples.

Conclusions:

The developed multifunctional DNA nanonet-based biosensor offers a highly sensitive and self-powered platform for tobramycin detection.
The integration of advanced nanomaterials and enzyme biofuel cells provides a robust system for real-time monitoring.
This technology holds promise for clinical diagnostics and therapeutic drug monitoring.