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Cyclophilin D reduces Ca2+ sequestration by complement 1q binding protein.

Oluwatobi Adegbite1,2, Yetunde Adegbite1, Catrin Pickering1

  • 1Institute of Systems and Molecular Biology, Biosciences Building, University of Liverpool, Institute of Systems and Molecular Biology, Biosciences Building University of Liverpool, Liverpool L69 7ZB, U.K.

The Biochemical Journal
|December 16, 2025
PubMed
Summary
This summary is machine-generated.

Complement 1q binding protein (C1qBP) acts as a mitochondrial calcium chelator, but its efficiency is reduced when bound to cyclophilin D (CypD). This interaction may explain CypD’s role in mitochondrial permeability transition pore opening.

Keywords:
C1qBPcalciumcyclophilin Dmitochondriapermeability transition pore

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Area of Science:

  • Mitochondrial biology
  • Protein-protein interactions
  • Calcium signaling

Background:

  • Complement 1q binding protein (C1qBP) regulates oxidative phosphorylation.
  • Cyclophilin D (CypD) regulates the mitochondrial permeability transition pore (mPTP).
  • Both C1qBP and CypD are mitochondrial matrix proteins.

Purpose of the Study:

  • To investigate the interaction between C1qBP and CypD.
  • To elucidate the functional consequences of this interaction on C1qBP's calcium-binding capacity.
  • To explore the potential role of this interaction in mPTP regulation.

Main Methods:

  • In vitro protein-protein binding assays.
  • Cyclosporin A treatment and site-directed mutagenesis of CypD.
  • AlphaFold protein modeling.
  • pH-dependent binding studies.
  • Calcium-binding assays.

Main Results:

  • C1qBP and CypD form a stable complex in vitro.
  • The interaction is disrupted by cyclosporin A and CypD active site mutations.
  • AlphaFold modeling suggests electrostatic attraction between C1qBP and CypD.
  • CypD binding reduces C1qBP's calcium-binding capacity and conformational changes.
  • C1qBP functions as a mitochondrial calcium chelator, with efficiency reduced by CypD.

Conclusions:

  • C1qBP acts as a mitochondrial calcium chelator.
  • CypD binding diminishes C1qBP's calcium-chelating ability.
  • CypD and Ca2+ likely compete for the same binding site on C1qBP.
  • The C1qBP-CypD interaction may be functionally relevant to Ca2+-dependent mPTP opening.