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Circular RNA circSCMH1 regulates glycolysis to inhibit gastric cancer metastasis via miR-296-3p/HSPB7-GLUT3 axis

Yue Yang1, Qiushuang Wang2, Zhe Gong3

  • 1Department of Medical Oncology, Fudan University Shanghai Cancer Center, Shanghai, 200032, PR China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, PR China; Department of Medical Oncology, Huadong Hospital Affiliated to Fudan University, Shanghai, 200040, PR China.

Translational Oncology
|December 16, 2025
PubMed

Insights

Circular RNAs (circRNAs) like circSCMH1 are crucial in gastric cancer (GC) metastasis. Lower circSCMH1 expression correlates with higher metastasis, suggesting it as a potential therapeutic target for GC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gastric cancer (GC) metastasis is a primary cause of cancer-related mortality.
  • Circular RNAs (circRNAs) are emerging as critical regulators in GC metastasis.
  • Identifying key molecules involved in GC metastasis remains a challenge.

Purpose of the Study:

  • To identify metastasis-driven circRNAs in gastric cancer.
  • To elucidate the biological function and mechanism of circSCMH1 in GC metastasis.
  • To investigate the clinical significance of circSCMH1 in GC patients.

Main Methods:

  • Next-generation sequencing to identify metastasis-associated circRNAs.
  • In vivo and in vitro experiments to determine circSCMH1 function.
  • Luciferase reporter assays and functional rescue tests to identify molecular interactions.
  • Glycolysis assays (glucose uptake, lactate, ATP production).
  • Clinical association analysis in 110 GC samples.

Main Results:

  • circSCMH1 expression was significantly lower in high-metastasis GC tissues.
  • circSCMH1 suppressed GC proliferation and metastasis by inhibiting glycolysis.
  • The mechanism involves the circSCMH1/miR-296-3p/HSPB7-GLUT3 axis.
  • Lower circSCMH1 expression was associated with lymph node metastasis and poor prognosis.

Conclusions:

  • circSCMH1 inhibits gastric cancer metastasis by regulating glycolysis via the miR-296-3p/HSPB7-GLUT3 pathway.
  • circSCMH1 serves as a potential biomarker for GC lymph node metastasis.
  • circSCMH1 represents a promising therapeutic target for gastric cancer.
Keywords:
Circular RNAGastric cancerGlycolysisMetastasisprognosis

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