Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservation of Protein Domains Over Different Proteins

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

LC-MS profiling of <i>prmt-1</i> and <i>prmt-5</i> knockout <i>C. elegans</i> reveals PRMT-1 substrates and global proteome remodeling.

bioRxiv : the preprint server for biology·2026

Same author

Systematic Identification of Germ Granule Proteins Reveals Specialized Roles in RNAi and Small RNA Inheritance.

bioRxiv : the preprint server for biology·2026

Same author

The ubiquitin-proteasome pathway mediates selective degradation of unloaded Argonaute proteins in C. elegans.

Genetics·2026

Same author

RG motifs promote piRNA-mediated gene silencing in C. elegans.

Nucleic acids research·2025

Same author

Argonaute-siRNA loading via the RNA-binding protein RDE-4 in C. elegans.

bioRxiv : the preprint server for biology·2025

Same author

RG motifs promote piRNA-mediated gene silencing in <i>C. elegans</i>.

bioRxiv : the preprint server for biology·2025

Same journal

Optimized tRNA structure-seq reveals robust tRNA secondary structures in <i>S. cerevisiae</i> under mild stress conditions.

RNA (New York, N.Y.)·2026

Same journal

SERIPH: A Two-Step Extraction Protocol for Selective Enrichment of Semi-Extractable RNAs.

RNA (New York, N.Y.)·2026

Same journal

Reduced Sensitivity to RNA Structural Differences Distinguishes Eukaryotic Pus4 from Bacterial TruB.

RNA (New York, N.Y.)·2026

Same journal

Puf3 contributes to changes in mRNA solubility, translation elongation dynamics at rare arginine codons and loss of protein homeostasis in cells lacking Not4.

RNA (New York, N.Y.)·2026

Same journal

RBM38 Regulates HORMAD1 Splicing to Enhances MEK Inhibitor Sensitivity in Breast Cancer.

RNA (New York, N.Y.)·2026

Same journal

EF-P Inhibits Ribosomal α-Hydroxy Acid Incorporation: Strategic tRNA Body Selection for Co-incorporating α-Hydroxy Acids and Nonproteinogenic Amino Acids into Depsipeptides.

RNA (New York, N.Y.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 8, 2026

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Published on: May 23, 2020

Decoding Argonaute specificity: insights from C. elegans and beyond.

Shihui Chen¹, Carolyn Marie Phillips²

¹Department of Biological Sciences, University of Southern California, Los Angeles, California 90089-2910, USA.

RNA (New York, N.Y.)

|December 16, 2025

Summary

This summary is machine-generated.

Small noncoding RNAs guide gene silencing by loading into Argonaute proteins. This review explores the rules governing this precise RNA loading, crucial for RNA interference (RNAi) specificity.

Keywords:

Argonaute C. elegans RNAi gene regulation small RNA

More Related Videos

Automated Analysis of C. elegans Fluorescence Images using SegElegans

Automated Analysis of C. elegans Fluorescence Images using SegElegans

Published on: October 10, 2025

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 25, 2013

Related Experiment Videos

Last Updated: Jan 8, 2026

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Protein Extract Preparation and Co-immunoprecipitation from Caenorhabditis elegans

Published on: May 23, 2020

Automated Analysis of C. elegans Fluorescence Images using SegElegans

Automated Analysis of C. elegans Fluorescence Images using SegElegans

Published on: October 10, 2025

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Large-scale Gene Knockdown in C. elegans Using dsRNA Feeding Libraries to Generate Robust Loss-of-function Phenotypes

Published on: September 25, 2013

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Small noncoding RNAs regulate gene expression by guiding the silencing of messenger RNAs (mRNAs) via Argonaute proteins.
RNA interference (RNAi) is a conserved mechanism essential for biological processes, relying on specific small RNA-Argonaute interactions for target recognition.

Purpose of the Study:

To synthesize current knowledge on the specificity of small RNA loading into Argonaute proteins.
To investigate the factors influencing selective Argonaute loading, using Caenorhabditis elegans as a model system.

Main Methods:

Review of existing literature on small RNA biogenesis and Argonaute protein interactions.
Integration of findings from Caenorhabditis elegans with data from other organisms.
Analysis of biochemical properties, structural features, post-translational modifications, and expression patterns.

Main Results:

Small RNA loading specificity is orchestrated by a complex interplay of factors.
Biogenesis, RNA properties, Argonaute structure, modifications, and expression patterns collectively ensure precise loading.
Caenorhabditis elegans offers a valuable model for studying these intricate loading mechanisms.

Conclusions:

Understanding Argonaute loading specificity is key to deciphering RNAi pathway fidelity.
Multiple layers of regulation ensure that the correct small RNAs associate with their cognate Argonaute partners.
Further research, particularly in model organisms like C. elegans, will illuminate the precise rules governing this fundamental biological process.