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Related Concept Videos

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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
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Effects of Spectral Interfering Substances on Light Transmission Platelet Aggregation Using Infrared Based

Manal Ibrahim-Kosta1, Gaïa Zirka1, Karine Carriere2

  • 1Laboratory of Hematology, Centre de Référence Des Pathologies Plaquettaires, C2VN, INRAE, INSERM, Aix Marseille Université, Marseille, France.

Journal of Clinical Laboratory Analysis
|December 17, 2025
PubMed
Summary
This summary is machine-generated.

Hemolyzed, icteric, or lipemic samples can interfere with platelet aggregation (PA) testing. Grossly hemolyzed samples (>0.6 g/L) should be rejected to ensure accurate PA results.

Keywords:
hemolysisicteruslight transmission platelet aggregationlipemiapreanalytical interference

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Area of Science:

  • Hematology
  • Clinical Chemistry
  • Diagnostic Assays

Background:

  • Platelet aggregation (PA) is a critical indicator of hemostasis.
  • Hemolyzed, icteric, or lipemic (HIL) samples can interfere with various laboratory tests.
  • The TA-8 V aggregometer utilizes a near-infrared light source to mitigate HIL interference.

Purpose of the Study:

  • To evaluate the impact of HIL interference on PA testing using the TA-8 V aggregometer.
  • To determine the interference thresholds for hemolysis, icterus, and lipemia in PA assays.

Main Methods:

  • Platelet-Rich-Plasma (PRP) samples were spiked with varying concentrations of red blood cell hemolysate (RBCH), bilirubin, and Intralipid.
  • Maximal intensity (MaxInt) and velocity (Vel) of platelet aggregation were measured in response to ADP and collagen.
  • The effect of apyrase on RBCH interference was also investigated.

Main Results:

  • Spontaneous platelet aggregation occurred at RBCH concentrations above 0.6 g/L.
  • Apyrase treatment of RBCH prevented spontaneous aggregation and reduced interindividual variability.
  • Increased RBCH concentrations significantly decreased MaxInt and Vel in response to ADP and collagen.
  • Bilirubin did not affect MaxInt or Vel.
  • Lipids significantly increased MaxInt at concentrations of 0.5 g/L and above.

Conclusions:

  • The TA-8 V aggregometer is not significantly affected by icterus or hyperlipidemia within tested ranges in healthy individuals.
  • Grossly hemolyzed samples (>0.6 g/L) interfere with PA testing due to released ADP and should be rejected.
  • Further studies are needed to validate these findings in patients with platelet dysfunction.