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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
Published on: October 7, 2025
312
AND-Gate Logic-Controlled Catalytic Hybridization Network for Robust Dual-Mode Fluorescence-Colorimetric Detection of
Yao Yao1, Shuyan Chen1, Yujun Cheng1
1College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan 410083, China.
Analytical Chemistry
|December 17, 2025
Summary
This study presents a novel dual-mode biosensor for accurate breast cancer detection using microRNAs (miRNAs). The innovative method enhances sensitivity and specificity for reliable liquid biopsy diagnostics.
Area of Science:
- Biomedical Engineering
- Molecular Diagnostics
- Nanotechnology
Background:
- Circulating microRNAs (miRNAs) are promising biomarkers for breast cancer, but their low abundance and complex serum matrices hinder sensitive detection.
- Existing methods for simultaneous miRNA detection often lack the required sensitivity and accuracy for clinical applications.
Purpose of the Study:
- To develop a dual-mode fluorescence-colorimetric biosensor for ultrasensitive and accurate profiling of breast cancer-associated miRNAs (miR-21 and miR-155).
- To establish a practical framework for multiplexed miRNA detection using dual-signal biosensors for noninvasive molecular diagnostics.
Main Methods:
- Utilized metal-organic framework composites (PCNPt) as signal converters for dual fluorescence and colorimetric readouts.
- Employed superparamagnetic Fe3O4@Au nanoparticles for specific enrichment and isolation of target miRNAs from serum.
- Developed an AND-gated response system triggering programmed assembly into a catalytic hybridization network (Magnetic PCNPt Net) for signal amplification.
Main Results:
- Achieved ultrasensitive and high-accuracy quantification of multiple miRNAs (miR-21 and miR-155) in clinical serum specimens.
- Demonstrated robust performance with reduced background interference due to magnetic enrichment.
- Validated the clinical applicability of the biosensor for breast cancer diagnostics through robust quantification.
Conclusions:
- The developed dual-mode biosensor offers a sensitive, specific, and accurate platform for multiplexed miRNA detection in liquid biopsies.
- This approach provides a practical framework for advancing noninvasive molecular diagnostics, particularly for breast cancer.
- The inherent self-calibration afforded by dual signaling significantly boosts analytical performance, paving the way for improved diagnostic tools.
