Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.3K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.3K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

25.6K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.6K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.8K
3.8K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.6K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

2.0K
2.0K
What is Gene Expression?01:36

What is Gene Expression?

10.8K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
10.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unexpected heterogeneity and tissue-specific properties of the thymic hematopoietic antigen-presenting cell network.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Regulation of actin cytoskeletal dynamics in T cell development and function.

Frontiers in immunology·2025
Same author

Evaluating <i>in vivo</i> approaches for studying the roles of thymic DCs in T cell development in mice.

Frontiers in immunology·2024
Same author

Nanoenabled intracellular zinc bursting for efficacious reversal of gefitinib resistance in lung cancer.

International journal of biological sciences·2024
Same author

Non-canonical RNA substrates of Drosha lack many of the conserved features found in primary microRNA stem-loops.

Scientific reports·2024
Same author

Distinct subpopulations of DN1 thymocytes exhibit preferential γδ T lineage potential.

Frontiers in immunology·2023

Related Experiment Video

Updated: Jan 8, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.2K

Regulation of the microprocessor by post-translational modifications.

Ka Weng Leong1,2, Mark M W Chong1,2

  • 1RNA and T cell Biology, St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia.

Frontiers in Cell and Developmental Biology
|December 17, 2025
PubMed
Summary

Post-translational modifications (PTMs) of the Microprocessor complex, including DROSHA and DGCR8, are crucial for microRNA biogenesis. This review explores how PTMs like phosphorylation and ubiquitination regulate Microprocessor function and stability.

Keywords:
DGCR8DROSHASUMOylationacetylationmicroRNA biogenesismicroprocessorphosphorylationubiquitination

More Related Videos

Author Spotlight: Enhancements in Gene Expression Regulation Research
07:10

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

2.5K
Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches
05:56

Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches

Published on: October 13, 2022

1.7K

Related Experiment Videos

Last Updated: Jan 8, 2026

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.2K
Author Spotlight: Enhancements in Gene Expression Regulation Research
07:10

Author Spotlight: Enhancements in Gene Expression Regulation Research

Published on: September 15, 2023

2.5K
Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches
05:56

Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches

Published on: October 13, 2022

1.7K

Area of Science:

  • Molecular Biology
  • Biochemistry

Background:

  • The Microprocessor complex, comprising DROSHA and DGCR8, initiates microRNA biogenesis.
  • Dysregulation of Microprocessor activity is linked to various diseases, including cancer and neurological disorders.

Purpose of the Study:

  • To review the known post-translational modifications (PTMs) of the Microprocessor complex.
  • To elucidate the functional roles of these PTMs in regulating Microprocessor activity and microRNA production.

Main Methods:

  • Literature review of studies on Microprocessor PTMs.
  • Focus on phosphorylation, acetylation, ubiquitination, and SUMOylation of DROSHA and DGCR8.

Main Results:

  • Multiple PTMs have been identified for DROSHA and DGCR8.
  • These modifications are implicated in regulating protein stability and microRNA processing.

Conclusions:

  • PTMs are critical regulators of Microprocessor function.
  • Further research is needed to fully characterize the functional impact of PTMs on microRNA biogenesis and associated diseases.