Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

592
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
592
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.6K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.6K
¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

1.7K
The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
1.7K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.0K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.0K
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule01:10

Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule

2.4K
In the AX proton spin system, proton A can sense the two spin states of a coupled proton X, resulting in a doublet NMR signal with two peaks of equal (1:1) intensity. When proton A is coupled to two equivalent protons (AX2 spin system), the spin states of each X can be aligned with or against the external field, creating three possible scenarios. This results in a 1:2:1  triplet signal, where the central peak corresponds to the chemical shift of A and is twice as large or intense as the...
2.4K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

665
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
665

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultra-Wideline 2D Correlations Among Low-γ Species in Solid-State NMR via the Progressive Saturation of a Common Proton Reservoir.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

On the effects of hyperpolarized water-based dissolution on the solute and solvent <sup>1</sup>H NMR spectra of small molecules.

Physical chemistry chemical physics : PCCP·2026
Same author

14.1 T Liquid-State <sup>19</sup>F Overhauser Dynamic Nuclear Polarization in an Analytical Organic Setting.

Journal of the American Chemical Society·2026
Same author

Assessing the treatment of pancreatic ductal adenocarcinoma by deuterium metabolic imaging: a preclinical study.

Magma (New York, N.Y.)·2026
Same author

Water Proton Spin Relaxivities and Absolute Fluorescent Quantum Yields of Triply and Quadruply Mixed Lanthanide Oxide Nanoparticles.

International journal of molecular sciences·2026
Same author

The ALS-associated E425K mutation uncouples DNAJC7 from the Hsp70 chaperone cycle.

The FEBS journal·2026
Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026
Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026
Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026
Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026
Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jan 8, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.6K

Enhanced Sensitivity and Resolution in Biomolecular CEST NMR Experiments Using the Extended Hadamard Encoding Scheme.

Jihyun Kim1,2, Micael Silva3, E̅riks Kupče4

  • 1Departments of Chemical and Biological Physics, Weizmann Institute of Science, Rehovot 7610001, Israel.

Analytical Chemistry
|December 17, 2025
PubMed
Summary
This summary is machine-generated.

The Hadamard Transform (HT) can accelerate Nuclear Magnetic Resonance (NMR) experiments but causes artifacts in Chemical Exchange Saturation Transfer (CEST) imaging. An extended HT (eHT) scheme eliminates these artifacts, improving CEST NMR performance.

More Related Videos

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.9K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.2K

Related Experiment Videos

Last Updated: Jan 8, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.6K
Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

15.9K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.2K

Area of Science:

  • Nuclear Magnetic Resonance (NMR) Spectroscopy
  • Chemical Exchange Saturation Transfer (CEST) Imaging

Background:

  • Hadamard Transform (HT) enables faster Nuclear Magnetic Resonance (NMR) experiments by simplifying spectral information extraction.
  • However, complex interactions like spin-spin couplings and chemical exchange can disrupt the direct link between spectral peak intensity and frequency, leading to artifacts in NMR experiments.

Purpose of the Study:

  • To identify and explain the origin of artifacts in NMR Chemical Exchange Saturation Transfer (CEST) experiments when using frequency-domain Hadamard encodings.
  • To propose and validate an extended Hadamard Transform (eHT) scheme to eliminate these artifacts while retaining the benefits of HT.

Main Methods:

  • Investigated artifacts arising from classical frequency-domain Hadamard encodings in NMR CEST experiments.
  • Developed and implemented an extended Hadamard Transform (eHT) scheme.
  • Performed and processed CEST NMR experiments using the eHT scheme.

Main Results:

  • Demonstrated that classical frequency-domain Hadamard encodings introduce significant artifacts in NMR CEST experiments due to chemical exchange.
  • The proposed eHT scheme successfully eliminated these artifacts in CEST NMR experiments.
  • eHT-processed CEST NMR experiments showed improved performance compared to standard implementations and other accelerated methods for protein CEST.

Conclusions:

  • The extended Hadamard Transform (eHT) effectively removes artifacts in NMR CEST experiments caused by classical Hadamard encodings.
  • eHT preserves the sensitivity and speed advantages of the Hadamard Transform for accelerated CEST NMR.
  • This advancement offers a more robust and accurate approach for advanced protein CEST NMR studies.