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Related Experiment Video

Updated: Jan 8, 2026

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
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DNA-Guided Robust Single-Protein Electronic Readout.

Ziyi Ju1,2, Zhaoxiang Deng1,2, Yueqi Li1

  • 1Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, 230026, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|December 17, 2025
PubMed
Summary
This summary is machine-generated.

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Researchers developed a DNA origami method for precise single-protein electrical measurements. This technique controls protein orientation, reducing variability and enabling detection of subtle changes upon molecule binding.

Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Electronics

Background:

  • Single-protein electronic transport offers insights into molecular function.
  • Variability in protein orientation hinders precise electrical measurements.

Purpose of the Study:

  • To develop a DNA origami method for controlled protein orientation.
  • To enable reproducible single-molecule electrical characterization.
  • To detect molecular binding events through electrical changes.

Main Methods:

  • Utilized DNA origami nanocavities with orthogonal aptamers for protein capture.
  • Employed matrix-patterned current-voltage (I-V) measurements.
  • Applied the method to thrombin and streptavidin proteins.
Keywords:
DNA origamibiosensingprotein conductance measurementsingle‐molecular conductance

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Last Updated: Jan 8, 2026

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Main Results:

  • Achieved preferred protein orientation, significantly reducing conductance variability.
  • Successfully detected conductance changes in thrombin upon binding with Na+ or inhibitors.
  • Demonstrated the platform's generalizability with streptavidin and selective detection in mixed samples.

Conclusions:

  • The DNA origami platform enables deterministic protein orientation control for electronic measurements.
  • This approach enhances reproducibility and sensitivity in single-protein electronics.
  • Highlights the potential of DNA nanotechnology for advanced molecular interfacing.